PRPF4 Antibody - #DF12707
| 製品: | PRPF4 Antibody |
| カタログ: | DF12707 |
| タンパク質の説明: | Rabbit polyclonal antibody to PRPF4 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 58 kDa; 58kD(Calculated). |
| ユニプロット: | O43172 |
| RRID: | AB_2845668 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12707, RRID:AB_2845668.
折りたたみ／展開
hPrp4; HPRP4P; PRP4; PRP4 homolog; PRP4 pre mRNA processing factor 4 homolog (yeast); PRP4/STK/WD splicing factor; PRP4_HUMAN; Prp4p; PRPF4; RP70; SNRNP60; U4/U6 small nuclear ribonucleoprotein Prp4; U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4;
免疫原
- O43172 PRP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASSRASSTQATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKDGLKAGIEAGNINITSGEVFEIEEHISERQAEVLAEFERRKRARQINVSTDDSEVKACLRALGEPITLFGEGPAERRERLRNILSVVGTDALKKTKKDDEKSKKSKEEYQQTWYHEGPNSLKVARLWIANYSLPRAMKRLEEARLHKEIPETTRTSQMQELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWDLEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYHIATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43172 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K15 | Sumoylation | Uniprot | |
| K27 | Acetylation | Uniprot | |
| K27 | Sumoylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| K45 | Sumoylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K53 | Acetylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K146 | Acetylation | Uniprot | |
| K147 | Acetylation | Uniprot | |
| K159 | Sumoylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| Y162 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| R345 | Methylation | Uniprot | |
| K456 | Acetylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| K486 | Ubiquitination | Uniprot |
研究背景
Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
Nucleus. Nucleus speckle.
Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. Interacts directly with PRPF18, PPIH and PRPF3. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. Interacts with ERCC6.
研究領域
· Genetic Information Processing > Transcription > Spliceosome.
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