PSPH Antibody - #DF12711

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製品: PSPH Antibody
カタログ: DF12711
タンパク質の説明: Rabbit polyclonal antibody to PSPH
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 25 kDa; 25kD(Calculated).
ユニプロット: P78330
RRID: AB_2845672

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(92%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
PSPH Antibody detects endogenous levels of total PSPH.
RRID:
AB_2845672
引用形式: Affinity Biosciences Cat# DF12711, RRID:AB_2845672.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EC 3.1.3.3; L 3 phosphoserine phosphatase; L-3-phosphoserine phosphatase; O phosphoserine phosphohydrolase; O-phosphoserine phosphohydrolase; Phosphoserine phosphatase; Phosphoserine phosphatase deficiency, included; PSP; PSPase; Psph; PSPHD; SERB_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human PSPH, corresponding to a region within the internal amino acids.

Uniprot:

P78330(SERB_HUMAN) >>Visit HPA database.

遺伝子(ID):
PSPH(5723)
タンパク質配列:
MVSHSELRKLFYSADAVCFDVDSTVIREEGIDELAKICGVEDAVSEMTRRAMGGAVPFKAALTERLALIQPSREQVQRLIAEQPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNIPATNVFANRLKFYFNGEYAGFDETQPTAESGGKGKVIKLLKEKFHFKKIIMIGDGATDMEACPPADAFIGFGGNVIRQQVKDNAKWYITDFVELLGELEE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Chicken
100
Horse
92
Dog
92
Xenopus
92
Rabbit
92
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates.

タンパク質ファミリー:

Belongs to the HAD-like hydrolase superfamily. SerB family.

研究領域

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

参考文献

1). Chemotherapy-induced macrophage CXCL7 expression drives tumor chemoresistance via the STAT1/PHGDH-serine metabolism axis and SAM paracrine feedback to M2 polarization. Cell death & disease, 2025 (PubMed: 40368902) [IF=8.1]

Application: WB    Species: human    Sample:

Fig. 4: CXCL7 upregulates PHGDH and activates serine metabolism in cancer cells. A Left: GSEA of enriched pathways in differentially expressed genes (DEGs) between HT29-control and HT29-CXCL7 groups. Right: Comparative analysis of PHGDH FPKM values between groups. B The expression level of PHGDH in control and CXCL7-overexpressing cells with or without the CXCR2 inhibitor, as determined by RT-qPCR. C Western blot detection of serine biosynthesis enzymes (PHGDH, PSAT1, PSPH).GAPDH was used as the loading control. D Kaplan‒Meier analysis of overall survival in TCGA-CRC patients stratified by PHGDH expression. E Left: Metabolite enrichment pathway bubble plot. Middle: Heatmap of differential metabolites. Right: Schematic of serine/one-carbon metabolism. F Heatmap visualization of DEGs in glycine, serine, and threonine metabolism pathways. G ELISA analysis of SAM levels in CXCL7-overexpressing cells and PHGDH-inhibited cells. H TUNEL assays of CXCL7-overexpressing cells transfected with NC-siRNA or PHGDH-siRNA, with or without SAM treatment(scale bar, 50 μm). I The drug sensitivity of CXCL7-overexpressing cells transfected with NC-siRNA or PHGDH-siRNA, with or without SAM treatment. All data are presented as mean ± SD. *P 
2). Serine Metabolism Regulates the Replicative Senescence of Human Dental Pulp Cells through Histone Methylation. Current issues in molecular biology, 2024 (PubMed: 38666909) [IF=2.8]

Application: WB    Species: human    Sample: hDPCs

Figure 2. PHGDH is involved in the cellular senescence of hDPCs. (a) Analysis of the transcription of PHGDH, PSAT1, and PSPH in different passages (P5 and P12) of hDPCs by RT-qPCR. (b) Analysis of PHGDH, PSAT1, and PSPH expression in different passages (P5 and P12) of hDPCs by western blot. (c) Young hDPCs (P5) were treated with different concentrations of NCT—503 and CBR—5884 for 48 h, and cell viability was determined by CCK8 assay. (d) Effects of NCT—503 (20 μM) and CBR—5884 (30 μM) treatment for 48 h on young hDPCs (P5) were determined by SA-β-gal staining, Ki67 staining, and yH2AX staining. In SA-β-gal staining, blue-stained cells are positive cells. In Ki67 staining and yH2AX staining, the blue color in the nucleus shows DAPI staining, the green color in the nucleus shows Ki67 or yH2AX staining. (e) Transcription of LMNB1 and P21 in hDPCs (P5) treated with NCT—503 (20 μM) and CBR—5884 (30 μM) for 48 h was analyzed by RT-qPCR. (f) Crystal violet staining determined colony formation at 7 days after continuous treatment with NCT—503 (20 μM) and 2 days of CBR—5884 (30 μM) treatment. Data were expressed as means ± SD, n = 3 independent experiments. * p < 0.05.

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