Ribophorin I Antibody - #DF12727
| 製品: | Ribophorin I Antibody |
| カタログ: | DF12727 |
| タンパク質の説明: | Rabbit polyclonal antibody to Ribophorin I |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat, Monkey |
| 予測: | Pig, Bovine, Horse, Rabbit, Dog, Xenopus |
| 分子量: | 69 kDa; 69kD(Calculated). |
| ユニプロット: | P04843 |
| RRID: | AB_2845688 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12727, RRID:AB_2845688.
折りたたみ／展開
D6Wsu137e; Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit; Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; EC 2.4.1.119; Oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); OST1; RBPH1; Ribophorin I; Ribophorin-1; RPN-I; Rpn1; RPN1_HUMAN;
免疫原
- P04843 RPN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAPAAGLFLLLLLGTWAPAPGSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYALKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRAPDELHYTYLDTFGRPVIVAYKKNLVEQHIQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRIGLYRHFDETVNRYKQSRDISTLNSGKKSLETEHKALTSEIALLQSRLKTEGSDLCDRVSEMQKLDAQVKELVLKSAVEAERLVAGKLKKDTYIENEKLISGKRQELVTKIDHILDAL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P04843 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K37 | Ubiquitination | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| Y135 | Phosphorylation | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| K187 | Acetylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| Y209 | Phosphorylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| Y264 | Phosphorylation | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| Y293 | Phosphorylation | Uniprot | |
| N299 | N-Glycosylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| Y398 | Phosphorylation | Uniprot | |
| T399 | Phosphorylation | Uniprot | |
| K413 | Acetylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K414 | Acetylation | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| C477 | S-Nitrosylation | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| K516 | Acetylation | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| K524 | Acetylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| K538 | Acetylation | Uniprot | |
| K538 | Sumoylation | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| R547 | Methylation | Uniprot | |
| K553 | Acetylation | Uniprot | |
| K553 | Ubiquitination | Uniprot | |
| K559 | Ubiquitination | Uniprot | |
| K564 | Ubiquitination | Uniprot | |
| K576 | Ubiquitination | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| K587 | Acetylation | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| T598 | Phosphorylation | Uniprot | |
| K599 | Ubiquitination | Uniprot |
研究背景
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Endoplasmic reticulum. Endoplasmic reticulum membrane>Single-pass type I membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Expressed in all tissues tested.
Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).
Belongs to the OST1 family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
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