SPTLC1 Antibody - #DF12752
(1)
(4)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12752, RRID:AB_2845713.
折りたたみ／展開
HSAN; HSAN1; HSN1; LBC1; LCB 1; LCB1; Long chain base biosynthesis protein 1; MGC14645; Serine C palmitoyltransferase; Serine palmitoyl CoA transferase 1; Serine palmitoyltransferase 1; Serine palmitoyltransferase long chain base subunit 1; Serine palmitoyltransferase subunit 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1; SPTC1_HUMAN; SPTI; SPTLC 1; SPTLC1;
免疫原
- O15269 SPTC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATATEQWVLVEMVQALYEAPAYHLILEGILILWIIRLLFSKTYKLQERSDLTVKEKEELIEEWQPEPLVPPVPKDHPALNYNIVSGPPSHKTVVNGKECINFASFNFLGLLDNPRVKAAALASLKKYGVGTCGPRGFYGTFDVHLDLEDRLAKFMKTEEAIIYSYGFATIASAIPAYSKRGDIVFVDRAACFAIQKGLQASRSDIKLFKHNDMADLERLLKEQEIEDQKNPRKARVTRRFIVVEGLYMNTGTICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRGVTEHYGINIDDIDLISANMENALASIGGFCCGRSFVIDHQRLSGQGYCFSASLPPLLAAAAIEALNIMEENPGIFAVLKEKCGQIHKALQGISGLKVVGESLSPAFHLQLEESTGSREQDVRLLQEIVDQCMNRSIALTQARYLEKEEKCLPPPSIRVVVTVEQTEEELERAASTIKEVAQAVLL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O15269 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K45 | Ubiquitination | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| T53 | Phosphorylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| Y164 | Phosphorylation | P00519 (ABL1) | Uniprot |
| K197 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K210 | Methylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| K434 | Acetylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| C438 | S-Nitrosylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| K465 | Ubiquitination | Uniprot |
研究背景
Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis (By similarity).
Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.
Endoplasmic reticulum membrane>Single-pass membrane protein.
Widely expressed. Not detected in small intestine.
Heterodimer with SPTLC2 or SPTLC3. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. The composition of the complex will define the substrate specificity. Interacts with SPTSSA and SPTSSB; the interaction is direct. Interacts with ORMDL3. Interacts with RTN4 (isoform B) (By similarity).
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
研究領域
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Metabolism > Lipid metabolism > Sphingolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
参考文献
Application: WB Species: Mice Sample: H9C2 cells
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