TRPC1 Antibody - #DF12783

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製品: TRPC1 Antibody
カタログ: DF12783
タンパク質の説明: Rabbit polyclonal antibody to TRPC1
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 91 kDa; 91kD(Calculated).
ユニプロット: P48995
RRID: AB_2845744

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(83%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
TRPC1 Antibody detects endogenous levels of total TRPC1.
RRID:
AB_2845744
引用形式: Affinity Biosciences Cat# DF12783, RRID:AB_2845744.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

HTRP 1; HTRP1; MGC133334; MGC133335; Mtrp1; Short transient receptor potential channel 1; Transient receptor potential canonical 1; Transient receptor potential cation channel subfamily C member 1; Transient receptor potential channel 1; Transient receptor protein 1; TRP 1; TRP 1 protein; Trp related protein 1; TRP-1; TRP1; TRP1 protein; TrpC1; Trpc1 transient receptor potential cation channel subfamily C member 1; TRPC1_HUMAN; Trrp1;

免疫原

免疫原:
Uniprot:

P48995(TRPC1_HUMAN) >>Visit HPA database.

遺伝子(ID):
TRPC1(7220)
発現特異性:
P48995 TRPC1_HUMAN:

Seems to be ubiquitous.

タンパク質配列:
MMAALYPSTDLSGASSSSLPSSPSSSSPNEVMALKDVREVKEENTLNEKLFLLACDKGDYYMVKKILEENSSGDLNINCVDVLGRNAVTITIENENLDILQLLLDYGCQSADALLVAIDSEVVGAVDILLNHRPKRSSRPTIVKLMERIQNPEYSTTMDVAPVILAAHRNNYEILTMLLKQDVSLPKPHAVGCECTLCSAKNKKDSLRHSRFRLDIYRCLASPALIMLTEEDPILRAFELSADLKELSLVEVEFRNDYEELARQCKMFAKDLLAQARNSRELEVILNHTSSDEPLDKRGLLEERMNLSRLKLAIKYNQKEFVSQSNCQQFLNTVWFGQMSGYRRKPTCKKIMTVLTVGIFWPVLSLCYLIAPKSQFGRIIHTPFMKFIIHGASYFTFLLLLNLYSLVYNEDKKNTMGPALERIDYLLILWIIGMIWSDIKRLWYEGLEDFLEESRNQLSFVMNSLYLATFALKVVAHNKFHDFADRKDWDAFHPTLVAEGLFAFANVLSYLRLFFMYTTSSILGPLQISMGQMLQDFGKFLGMFLLVLFSFTIGLTQLYDKGYTSKEQKDCVGIFCEQQSNDTFHSFIGTCFALFWYIFSLAHVAIFVTRFSYGEELQSFVGAVIVGTYNVVVVIVLTKLLVAMLHKSFQLIANHEDKEWKFARAKLWLSYFDDKCTLPPPFNIIPSPKTICYMISSLSKWICSHTSKGKVKRQNSLKEWRNLKQKRDENYQKVMCCLVHRYLTSMRQKMQSTDQATVENLNELRQDLSKFRNEIRDLLGFRTSKYAMFYPRN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
83
Bovine
75
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48995 基板として

Site PTM Type Enzyme
S206 Phosphorylation
T229 Phosphorylation
S279 Phosphorylation
T289 Phosphorylation
S308 Phosphorylation
T347 Phosphorylation
Y368 Phosphorylation
T415 Phosphorylation
Y425 Phosphorylation
S752 Phosphorylation
T757 Phosphorylation

研究背景

機能:

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.

PTMs:

Activation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells.

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Seems to be ubiquitous.

サブユニット構造:

Interacts with TRPC4AP (By similarity). Homotetramer and heterotetramer with TRPC4 and/or TRPC5. Interacts with TRPC3, TRPC4 and TRPC5. Interacts with ITPR3. Interacts with MX1 and RNF24. Interacts with FKBP4.

タンパク質ファミリー:

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.

研究領域

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Digestive system > Pancreatic secretion.

参考文献

1). Targeting transient receptor potential canonical 1 reduces non‑small cell lung cancer chemoresistance and stemness via inhibition of PI3K/AKT signaling. Oncology Letters, 2023 (PubMed: 37153044) [IF=2.9]

Application: WB    Species: Human    Sample: NSCLC cells

Figure 1. TRPC1 expression in normal and CDDP-resistant NSCLC cells. The dose-response curve of CDDP in (A) A549 cells, (B) A549/CDDP cells, (C) H460 cells, and (D) H460/CDDP cells. (E) TRPC1 mRNA expression. (F) Representative western blots of TRPC1 expression. (G) Densitometry analysis of TRPC1 protein expression. *P
2). HDAC1 regulates inflammation and osteogenic differentiation of ankylosing spondylitis fibroblasts through the Wnt-Smad signaling pathway. Journal of Orthopaedic Surgery and Research, 2022 (PubMed: 35794630) [IF=2.6]

Application: WB    Species: Human    Sample: AS fibroblasts

Fig. 4 Expression of TRPC1 and TRPV4 in AS fibroblasts after different treatment. A Protein expressions of TRPC1 and TRPV4 in AS fibroblasts were detected by western blot analysis. B Densitometry analysis of protein expression. C Representative images showing immunofluorescence staining of TRPC1. Magnification, × 400. D Representative images showing immunofluorescence staining of TRPV4. Magnification, × 400. E Mean fluorescence intensity of TRPC1. F Mean fluorescence intensity of TRPV4. Data were shown as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the AAV-NC group. #P < 0.05, ##P < 0.01 and ###P < 0.001, compared with the AAV-HDAC1 group. &P < 0.05, &&P < 0.01 and &&&P < 0.001, compared with the AAV-HDAC1 + DKK-1 group

Application: IF/ICC    Species: Human    Sample: AS fibroblasts

Fig. 4 Expression of TRPC1 and TRPV4 in AS fibroblasts after different treatment. A Protein expressions of TRPC1 and TRPV4 in AS fibroblasts were detected by western blot analysis. B Densitometry analysis of protein expression. C Representative images showing immunofluorescence staining of TRPC1. Magnification, × 400. D Representative images showing immunofluorescence staining of TRPV4. Magnification, × 400. E Mean fluorescence intensity of TRPC1. F Mean fluorescence intensity of TRPV4. Data were shown as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the AAV-NC group. #P < 0.05, ##P < 0.01 and ###P < 0.001, compared with the AAV-HDAC1 group. &P < 0.05, &&P < 0.01 and &&&P < 0.001, compared with the AAV-HDAC1 + DKK-1 group

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