VPS45 Antibody - #DF12796

(1)  
製品: VPS45 Antibody
カタログ: DF12796
タンパク質の説明: Rabbit polyclonal antibody to VPS45
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
分子量: 65 kDa; 65kD(Calculated).
ユニプロット: Q9NRW7
RRID: AB_2845757

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
VPS45 Antibody detects endogenous levels of total VPS45.
RRID:
AB_2845757
引用形式: Affinity Biosciences Cat# DF12796, RRID:AB_2845757.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

免疫原

免疫原:
Uniprot:

Q9NRW7(VPS45_HUMAN) >>Visit HPA database.

遺伝子(ID):
VPS45(11311)
発現特異性:
Q9NRW7 VPS45_HUMAN:

Ubiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver.

タンパク質配列:
MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEVLASGLHSRSKESSQVTSRSASRR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
92
Zebrafish
82
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NRW7 基板として

Site PTM Type Enzyme
Y10 Phosphorylation
K76 Ubiquitination
Y81 Phosphorylation
S168 Phosphorylation
K185 Ubiquitination
K192 Ubiquitination
K197 Ubiquitination
K257 Ubiquitination
Y272 Phosphorylation
K296 Ubiquitination
K304 Ubiquitination
S307 Phosphorylation
K312 Ubiquitination
Y318 Phosphorylation
K322 Methylation
K322 Ubiquitination
K323 Methylation
T327 Phosphorylation
K330 Ubiquitination
S339 Phosphorylation
K371 Ubiquitination
K378 Ubiquitination
K418 Ubiquitination
S424 Phosphorylation
K432 Ubiquitination
S437 Phosphorylation
S441 Phosphorylation
K443 Ubiquitination
K450 Ubiquitination
K454 Ubiquitination
K457 Ubiquitination
K479 Ubiquitination
K483 Ubiquitination
Y487 Phosphorylation
K557 Ubiquitination
S559 Phosphorylation
S560 Phosphorylation
T563 Phosphorylation
S564 Phosphorylation

研究背景

機能:

May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.

細胞の位置付け:

Golgi apparatus membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein.
Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver.

サブユニット構造:

Interacts with STX6 (By similarity). Interacts with ZFYVE20.

タンパク質ファミリー:

Belongs to the STXBP/unc-18/SEC1 family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.