ADIPOR2 Antibody - #DF12811

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製品: ADIPOR2 Antibody
カタログ: DF12811
タンパク質の説明: Rabbit polyclonal antibody to ADIPOR2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Dog
分子量: 50 kDa; 44kD(Calculated).
ユニプロット: Q86V24
RRID: AB_2845772

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HPLC Report
MSDS
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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(83%), Sheep(83%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ADIPOR2 Antibody detects endogenous levels of total ADIPOR2.
RRID:
AB_2845772
引用形式: Affinity Biosciences Cat# DF12811, RRID:AB_2845772.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

;PAQR2; ACDCR2; Adiponectin receptor protein 2; Adipor2; ADR2_HUMAN; Progestin and adipoQ receptor family member II;

免疫原

免疫原:
Uniprot:

Q86V24(PAQR2_HUMAN) >>Visit HPA database.

遺伝子(ID):
ADIPOR2(79602)
発現特異性:
Q86V24 PAQR2_HUMAN:

Ubiquitous (PubMed:16044242). Highly expressed in skeletal muscle, liver and placenta (PubMed:12802337). Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung (PubMed:12802337).

タンパク質配列:
MNEPTENRLGCSRTPEPDIRLRKGHQLDGTRRGDNDSHQGDLEPILEASVLSSHHKKSSEEHEYSDEAPQEDEGFMGMSPLLQAHHAMEKMEEFVCKVWEGRWRVIPHDVLPDWLKDNDFLLHGHRPPMPSFRACFKSIFRIHTETGNIWTHLLGCVFFLCLGIFYMFRPNISFVAPLQEKVVFGLFFLGAILCLSFSWLFHTVYCHSEGVSRLFSKLDYSGIALLIMGSFVPWLYYSFYCNPQPCFIYLIVICVLGIAAIIVSQWDMFATPQYRGVRAGVFLGLGLSGIIPTLHYVISEGFLKAATIGQIGWLMLMASLYITGAALYAARIPERFFPGKCDIWFHSHQLFHIFVVAGAFVHFHGVSNLQEFRFMIGGGCSEEDAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Bovine
83
Sheep
83
Chicken
75
Xenopus
73
Pig
0
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86V24 基板として

Site PTM Type Enzyme
K23 Ubiquitination
K56 Ubiquitination
S65 Phosphorylation
K90 Ubiquitination
K97 Ubiquitination
K116 Ubiquitination
K137 Ubiquitination

研究背景

機能:

Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal body fat and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels (By similarity).

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.
Note: Localized to the cell membrane and intracellular organelles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung.

サブユニット構造:

May form homooligomers and heterooligomers with ADIPOR1. Interacts with APPL2 (via BAR domain); ADIPOQ dissociates this interaction (By similarity).

タンパク質ファミリー:

The N-terminus is cytoplasmic and the C-terminus is extracellular, contrary to what is observed for G-protein coupled receptors. Unlike G-protein coupled receptors, transmembrane helices are not kinked or tilted relative to the plane of the membrane.

Belongs to the ADIPOR family.

研究領域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

参考文献

1). Atractylenolide III ameliorates Non-Alcoholic Fatty Liver Disease by activating Hepatic Adiponectin Receptor 1-Mediated AMPK Pathway. International Journal of Biological Sciences, 2022 (PubMed: 35280674) [IF=9.2]

Application: WB    Species: Mouse    Sample:

Figure 5 ATL III treatment restores the down-regulation of hepatic AdipoR1 expression and AdipoR1 signaling induced by HFD feeding in mice. A. Expression levels of hepatic AdipoR1 and AdipoR2 were examined in HFD mice. B. Relative levels of hepatic AdipoR1 and AdipoR2 were analyzed. C. AdipoR1 downstream signaling molecules including p-LKB1, p-AMPK, SIRT1, Nrf2, SIRT3, CPT1A and PGC1α were tested in HFD mice. D. Relative levels of p-LKB1, p-AMPK, SIRT1, Nrf2, SIRT3, CPT1A and PGC1α were analyzed.
2). Osteogenic effect of an adiponectin-derived short peptide that rebalances bone remodeling: a potential disease-modifying approach for postmenopausal osteoporosis therapy. Archives of pharmacal research, 2024 (PubMed: 39073743) [IF=6.7]
3). Effects of the Water Extract of Fermented Rice Bran on Liver Damage and Intestinal Injury in Aged Rats with High-Fat Diet Feeding. Plants, 2022 (PubMed: 35270077) [IF=4.5]

Application: WB    Species: Rat    Sample:

Figure 5 Effects of the water extract of fermented rice bran (FRB) on hepatic lipid metabolism-related protein expressions in aged rats with high-fat (HF) diet feeding. Values are presented as the mean ± standard deviation (n = 6). * p < 0.05 vs. the normal control (NC) group; significance between two groups was determined using Student’s t-test. In the HF diet-fed groups, significant differences between groups were determined by a one-way ANOVA with Fisher‘s post hoc test. Western blot analysis of (A) NAD-dependent sirtuin-1 (SIRT1), (B) adenosine monophosphate-activated protein kinase-α (AMPKα), (C) phosphorylated (p)-AMPKα, (D) p-AMPKα/AMPKα, (E) leptin receptor, and (F) adiponectin receptor 2 (AdipoR2) protein expressions. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) was used as an internal control. Quantitative analysis of protein levels and the ratio of each internal control were calculated by setting the value of the mean of the NC group.

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