AFMID Antibody - #DF12814
| 製品: | AFMID Antibody |
| カタログ: | DF12814 |
| タンパク質の説明: | Rabbit polyclonal antibody to AFMID |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| 分子量: | 30 kDa; 34kD(Calculated). |
| ユニプロット: | Q63HM1 |
| RRID: | AB_2845775 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12814, RRID:AB_2845775.
折りたたみ／展開
afmid; AFMID_HUMAN; KF; Kynurenine formamidase; Probable arylformamidase;
免疫原
- Q63HM1 KFA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMDVSGVGFPSKVPWKKMSAEELENQYCPSRWVVRLGAEEALRTYSQIGIEATTRARATRKSLLHVPYGDGEGEKVDIYFPDESSEALPFFLFFHGGYWQSGSKDESAFMVHPLTAQGVAVVIVAYGIAPKGTLDHMVDQVTRSVAFVQKRYPSNKGIYLCGHSAGAHLAAMMLLADWTKHGVTPNLRGFFLVSGVFDLEPIVYTSQNVALQLTLEDAQRNSPQLKVAQAQPVDPTCRVLVVVGQFDSPEFHRQSWEFYQTLCQGEWKASFEELHDVDHFEIVENLTQKDNVLTQIILKTIFQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q63HM1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y27 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot |
研究背景
Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.
Cytoplasm>Cytosol. Nucleus.
Note: Predominantly cytosolic. Some fraction is nuclear.
Homodimer.
The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.
Belongs to the kynurenine formamidase family.
研究領域
· Metabolism > Amino acid metabolism > Tryptophan metabolism.
· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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