AKAP8L Antibody - #DF12818
| 製品: | AKAP8L Antibody |
| カタログ: | DF12818 |
| タンパク質の説明: | Rabbit polyclonal antibody to AKAP8L |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 72 kDa; 72kD(Calculated). |
| ユニプロット: | Q9ULX6 |
| RRID: | AB_2845779 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12818, RRID:AB_2845779.
折りたたみ／展開
A kinase (PRKA) anchor protein 8 like; A kinase anchor protein 8 like; AKAP 8L; AKAP8 like protein; DKFZp434L0650; HA95; HAP95; Helicase A binding protein 95; Homologous to AKAP95 protein; NAKAP; NAKAP95; Neighbor of A kinase anchoring protein 95; Neighbor of AKAP95;
免疫原
- Q9ULX6 AKP8L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSYTGFVQGSETTLQSTYSDTSAQPTCDYGYGTWNSGTNRGYEGYGYGYGYGQDNTTNYGYGMATSHSWEMPSSDTNANTSASGSASADSVLSRINQRLDMVPHLETDMMQGGVYGSGGERYDSYESCDSRAVLSERDLYRSGYDYSELDPEMEMAYEGQYDAYRDQFRMRGNDTFGPRAQGWARDARSGRPMASGYGRMWEDPMGARGQCMSGASRLPSLFSQNIIPEYGMFQGMRGGGAFPGGSRFGFGFGNGMKQMRRTWKTWTTADFRTKKKKRKQGGSPDEPDSKATRTDCSDNSDSDNDEGTEGEATEGLEGTEAVEKGSRVDGEDEEGKEDGREEGKEDPEKGALTTQDENGQTKRKLQAGKKSQDKQKKRQRDRMVERIQFVCSLCKYRTFYEDEMASHLDSKFHKEHFKYVGTKLPKQTADFLQEYVTNKTKKTEELRKTVEDLDGLIQQIYRDQDLTQEIAMEHFVKKVEAAHCAACDLFIPMQFGIIQKHLKTMDHNRNRRLMMEQSKKSSLMVARSILNNKLISKKLERYLKGENPFTDSPEEEKEQEEAEGGALDEGAQGEAAGISEGAEGVPAQPPVPPEPAPGAVSPPPPPPPEEEEEGAVPLLGGALQRQIRGIPGLDVEDDEEGGGGAP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9ULX6 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y29 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| C128 | S-Nitrosylation | Uniprot | |
| R131 | Methylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| R171 | Methylation | Uniprot | |
| R179 | Methylation | Uniprot | |
| R185 | Methylation | Uniprot | |
| R188 | Methylation | Uniprot | |
| R191 | Methylation | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| R199 | Methylation | Uniprot | |
| R208 | Methylation | Uniprot | |
| R217 | Methylation | Uniprot | |
| R237 | Methylation | Uniprot | |
| R247 | Methylation | Uniprot | |
| K257 | Acetylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| T267 | Phosphorylation | Uniprot | |
| T268 | Phosphorylation | Uniprot | |
| R272 | Methylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| T292 | Phosphorylation | Uniprot | |
| T294 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| R397 | Methylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| Y435 | Phosphorylation | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| K500 | Acetylation | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| T504 | Phosphorylation | Uniprot | |
| K520 | Acetylation | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| K544 | Sumoylation | Uniprot | |
| K544 | Ubiquitination | Uniprot | |
| T550 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S601 | Phosphorylation | Uniprot |
研究背景
Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export. Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis. May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8. May be involved in regulation of pre-mRNA splicing.
(Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters.
(Microbial infection) Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses.
(Microbial infection) In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription; in vitro negatively regulates DHX9 annealing activity.
Phosphorylated on serine or threonine residues possibly by PKA; probably modulating the interaction with TMPO isoform Beta.
Nucleus. Nucleus matrix. Nucleus speckle. Nucleus>PML body. Cytoplasm.
Note: Colocalizes with PRPF40A in the nuclear matrix (PubMed:16391387). Nuclear at steady state but shuttles between the nucleus and cytoplasm (PubMed:10748171). The shuttling property has been questioned (PubMed:11034899). Colocalizes with EBNA-LP in PML bodies (PubMed:11884601).
Ubiquitously expressed. Expressed in the brain cortex (at protein level).
Interacts (via N-terminus) with DHX9 (via RGG region). Interacts with TMPO isoform Beta, PRPF40A, RNF43, lamin-B. Interacts with HDAC3; increased during mitosis. Interacts with EBV EBNA-LP. Interacts with HIV-1 reverse transcriptase/ribonuclease H.
Belongs to the AKAP95 family.
研究領域
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
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