製品: ARPC3 Antibody
カタログ: DF12839
タンパク質の説明: Rabbit polyclonal antibody to ARPC3
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 21 kDa; 21kD(Calculated).
ユニプロット: O15145
RRID: AB_2845800

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%)
クローナリティ:
Polyclonal
特異性:
ARPC3 Antibody detects endogenous levels of total ARPC3.
RRID:
AB_2845800
引用形式: Affinity Biosciences Cat# DF12839, RRID:AB_2845800.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Actin related protein 2/3 complex subunit 3; ARC21; ARP2/3 complex 21 kDa subunit; ARP2/3 protein complex subunit p21; ARPC3;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MPAYHSSLMDPDTKLIGNMALLPIRSQFKGPAPRETKDTDIVDEAIYYFKANVFFKNYEIKNEADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFPLNAIYAKPANKQEDEVMRAYLQQLRQETGLRLCEKVFDPQNDKPSKWWTCFVKRQFMNKSLSGPGQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
89
Zebrafish
67
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15145 基板として

Site PTM Type Enzyme
Y4 Phosphorylation
T13 Phosphorylation
K14 Ubiquitination
K29 Methylation
K29 Ubiquitination
K37 Acetylation
K37 Ubiquitination
Y47 Phosphorylation
Y48 Phosphorylation
K50 Acetylation
K56 Acetylation
K56 Ubiquitination
K61 Acetylation
K81 Ubiquitination
K123 Ubiquitination
K147 Acetylation
K147 Ubiquitination
K155 Acetylation
K155 Ubiquitination
K158 Ubiquitination
K165 Acetylation
K165 Ubiquitination
K171 Acetylation
K171 Ubiquitination

研究背景

機能:

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cell projection. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.

タンパク質ファミリー:

Belongs to the ARPC3 family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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For Research Use Only.
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