Phospho-BIK (Thr33) Antibody - #AF3428

(3)  
製品: Phospho-BIK (Thr33) Antibody
カタログ: AF3428
タンパク質の説明: Rabbit polyclonal antibody to Phospho-BIK (Thr33)
アプリケーション: WB IHC IF/ICC
反応性: Human
分子量: 30kDa; 18kD(Calculated).
ユニプロット: Q13323
RRID: AB_2834870

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説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
Phospho-BIK (Thr33) Antibody detects endogenous levels of BIK only when phosphorylated at Threonine 33.
RRID:
AB_2834870
引用形式: Affinity Biosciences Cat# AF3428, RRID:AB_2834870.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Apoptosis inducer NBK; BBC1; Bcl-2-interacting killer; BCL2 interacting killer; bhikhari; BIK; Bik-like killer protein; BIK_HUMAN; BIP 1; BiP1; BP 4; BP4; cb 60; NBK;

免疫原

免疫原:
Uniprot:

Q13323(BIK_HUMAN) >>Visit HPA database.

遺伝子(ID):
BIK(638)
タンパク質の説明:
The protein encoded by this gene is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins.
タンパク質配列:
MSEVRPLSRDILMETLLYEQLLEPPTMEVLGMTDSEEDLDPMEDFDSLECMEGSDALALRLACIGDEMDVSLRAPRLAQLSEVAMHSLGLAFIYDQTEDIRDVLRSFMDGFTTLKENIMRFWRSPNPGSWVSCEQVLLALLLLLALLLPLLSGGLHLLLK

PTMs - Q13323 基板として

Site PTM Type Enzyme
S2 Phosphorylation
T33 Phosphorylation P68400 (CSNK2A1)
S35 Phosphorylation P68400 (CSNK2A1)
K115 Ubiquitination
S124 Phosphorylation

研究背景

機能:

Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.

PTMs:

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage is a necessary step prior its degradation by the proteosome-dependent mechanism.

細胞の位置付け:

Endomembrane system>Single-pass membrane protein. Mitochondrion membrane>Single-pass membrane protein.
Note: Around the nuclear envelope, and in cytoplasmic membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with RHBDL4/RHBDD1.

タンパク質ファミリー:

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

研究領域

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

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