BRD8 Antibody - #DF12858
| 製品: | BRD8 Antibody |
| カタログ: | DF12858 |
| タンパク質の説明: | Rabbit polyclonal antibody to BRD8 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 120 kDa; 135kD(Calculated). |
| ユニプロット: | Q9H0E9 |
| RRID: | AB_2845819 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12858, RRID:AB_2845819.
折りたたみ／展開
BRD8; Bromodomain containing 8; p120; Skeletal muscle abundant protein; Skeletal muscle abundant protein 2; SMAP; SMAP2; Thyroid hormone receptor coactivating protein 120kDa; Thyroid hormone receptor coactivating protein;
免疫原
Expressed in adipose tissue, brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
- Q9H0E9 BRD8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATGTGKHKLLSTGPTEPWSIREKLCLASSVMRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSELLETTETPKRKRGEKGEVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDELCNDIATKKKLEEEEAEVKRKATDAAYQARQAVKTPPRRLPTVMVRSPIDSASPGGDYPLGDLTPTTMEEATSGVNESEMAVASGHLNSTGVLLEVGGVLPMIHGGEIQQTPNTVAASPAASGAPTLSRLLEAGPTQFTTPLASFTTVASEPPVKLVPPPVESVSQATIVMMPALPAPSSAPAVSTTESVAPVSQPDNCVPMEAVGDPHTVTVSMDSSEISMIINSIKEECFRSGVAEAPVGSKAPSIDGKEELDLAEKMDIAVSYTGEELDFETVGDIIAIIEDKVDDHPEVLDVAAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDFEETENKGIHELVDIREPSAEIKVEPAEPEPVISGAEIVAGVVPATSMEPPELRSQDLDEELGSTAAGEIVEADVAIGKGDETPLTNVKTEASPESMLSPSHGSNPIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAPGEDEEEDGVSEAASLEEPKEEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFSVCSEDQEAIQAQKIWKKAIMLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIRSTAEFQRDIMLMFQNAVMYNSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTRKQDASEKDSVPMGSPAFLLSLFMGHEWVWLDSEQDHPNDSELSNDCRSLFSSWDSSLDLDVGNWRETEDPEAEELEESSPEREPSELLVGDGGSEESQEAARKASHQNLLHFLSEVAYLMEPLCISSNESSEGCCPPSGTRQEGREIKASEGERELCRETEELSAKGDPLVAEKPLGENGKPEVASAPSVICTVQGLLTESEEGEAQQESKGEDQGEVYVSEMEDQPPSGECDDAFNIKETPLVDTLFSHATSSKLTDLSQDDPVQDHLLFKKTLLPVWKMIASHRFSSPFLKPVSERQAPGYKDVVKRPMDLTSLKRNLSKGRIRTMAQFLRDLMLMFQNAVMYNDSDHHVYHMAVEMRQEVLEQIQVLNIWLDKRKGSSSLEGEPANPVDDGKPVF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H0E9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T3 | Phosphorylation | Uniprot | |
| T5 | Phosphorylation | Uniprot | |
| K7 | Acetylation | Uniprot | |
| K9 | Acetylation | Uniprot | |
| T16 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T77 | Phosphorylation | Uniprot | |
| K85 | Acetylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| T163 | Phosphorylation | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| K481 | Acetylation | Uniprot | |
| K481 | Sumoylation | Uniprot | |
| R502 | Methylation | Uniprot | |
| K509 | Sumoylation | Uniprot | |
| T569 | Phosphorylation | Uniprot | |
| K575 | Sumoylation | Uniprot | |
| T576 | Phosphorylation | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| K612 | Sumoylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| S637 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| S656 | Phosphorylation | Uniprot | |
| S851 | Phosphorylation | Uniprot | |
| T1151 | Phosphorylation | Uniprot | |
| S1152 | Phosphorylation | Uniprot |
研究背景
May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.
Nucleus.
Expressed in adipose tissue, brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. BRD8 isoform 2 interacts with RXRA/NR2B1 and THRB/ERBA2 (By similarity). Component of a SWR1-like complex.
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