製品: CHMP1A Antibody
カタログ: DF12894
タンパク質の説明: Rabbit polyclonal antibody to CHMP1A
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 25 kDa; 22kD(Calculated).
ユニプロット: Q9HD42
RRID: AB_2845855

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(100%), Xenopus(82%)
クローナリティ:
Polyclonal
特異性:
CHMP1A Antibody detects endogenous levels of total CHMP1A.
RRID:
AB_2845855
引用形式: Affinity Biosciences Cat# DF12894, RRID:AB_2845855.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Charged multivesicular body protein 1a; CHM1A_HUMAN; CHMP1a; Chromatin modifying protein 1a; Chromatin-modifying protein 1a; hVps46 1; hVps46-1; KIAA0047; Metalloprotease 1; PCOLN3; Procollagen (type III) N endopeptidase; PRSM1; Vacuolar protein sorting 46 1; Vacuolar protein sorting-associated protein 46-1; Vps46 1; Vps46-1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9HD42 CHM1A_HUMAN:

Expressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG-63.

タンパク質配列:
MDDTLFQLKFTAKQLEKLAKKAEKDSKAEQAKVKKALLQKNVECARVYAENAIRKKNEGVNWLRMASRVDAVASKVQTAVTMKGVTKNMAQVTKALDKALSTMDLQKVSSVMDRFEQQVQNLDVHTSVMEDSMSSATTLTTPQEQVDSLIMQIAEENGLEVLDQLSQLPEGASAVGESSVRSQEDQLSRRLAALRN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
91
Xenopus
82
Zebrafish
73
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HD42 基板として

Site PTM Type Enzyme
M1 Acetylation
K13 Ubiquitination
K27 Acetylation
K40 Acetylation
K40 Ubiquitination
Y48 Phosphorylation
K56 Ubiquitination
S67 Phosphorylation
K75 Ubiquitination
K83 Ubiquitination
K87 Acetylation
K87 Ubiquitination
K94 Ubiquitination
K98 Ubiquitination
S101 Phosphorylation
S173 Phosphorylation
S178 Phosphorylation
S179 Phosphorylation
S182 Phosphorylation
S188 Phosphorylation

研究背景

機能:

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.

細胞の位置付け:

Cytoplasm. Endosome membrane>Peripheral membrane protein. Nucleus matrix.
Note: The cytoplasmic form is partially membrane-associated and localizes to early endosomes. The nuclear form remains associated with the chromosome scaffold during mitosis. On overexpression, it localizes to nuclear bodies characterized by nuclease-resistant condensed chromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG-63.

サブユニット構造:

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. Self-associates. Interacts with CHMP1B. Interacts with VPS4A. Interacts with VPS4B. Interacts with PHF1. Interacts with IST1. Interacts with MITD1.

タンパク質ファミリー:

Belongs to the SNF7 family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

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