CHMP1B Antibody - #DF12895
| 製品: | CHMP1B Antibody |
| カタログ: | DF12895 |
| タンパク質の説明: | Rabbit polyclonal antibody to CHMP1B |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Zebrafish, Bovine, Chicken, Xenopus |
| 分子量: | 28 kDa; 22kD(Calculated). |
| ユニプロット: | Q7LBR1 |
| RRID: | AB_2845856 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12895, RRID:AB_2845856.
折りたたみ／展開
2810405I11Rik; C10orf2; C18 ORF2; C18orf2; Charged multivesicular body protein 1b; CHM1B_HUMAN; CHMP family, member 1B; CHMP1.5; CHMP1b; Choromosome 18 open reading frame 2; Chromatin-modifying protein 1b; hVps46-2; vacuolar protein sorting 46-2; Vacuolar protein sorting-associated protein 46-2; Vps46-2; Vps46B;
免疫原
Widely expressed. Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta and brain.
- Q7LBR1 CHM1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSNMEKHLFNLKFAAKELSRSAKKCDKEEKAEKAKIKKAIQKGNMEVARIHAENAIRQKNQAVNFLRMSARVDAVAARVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFETLDVQTQQMEDTMSSTTTLTTPQNQVDMLLQEMADEAGLDLNMELPQGQTGSVGTSVASAEQDELSQRLARLRDQV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q7LBR1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| K16 | Acetylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| T102 | Phosphorylation | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot |
研究背景
Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells. Involved in HIV-1 p6- and p9-dependent virus release.
Cytoplasm>Cytosol. Endosome. Late endosome membrane>Peripheral membrane protein.
Note: Localizes to the midbody of dividing cells, colocalizing with CEP55 and CHMP5. Localized at the periphery of the Fleming body.
Widely expressed. Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta and brain.
Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. Interacts with CHMP1A. Interacts with VTA1; the interaction probably involves the open conformation of CHMP1B. Interacts with CHMP2A. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with SPAST (via MIT domain); the interaction is direct. Interacts with IST1. Interacts with MITD1. Interacts with STAMBP.
Belongs to the SNF7 family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
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