製品: DDA1 Antibody
カタログ: DF12930
タンパク質の説明: Rabbit polyclonal antibody to DDA1
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Dog, Chicken
分子量: 12 kDa; 12kD(Calculated).
ユニプロット: Q9BW61
RRID: AB_2845891

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
DDA1 Antibody detects endogenous levels of total DDA1.
RRID:
AB_2845891
引用形式: Affinity Biosciences Cat# DF12930, RRID:AB_2845891.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

C19orf58; Cross immune reaction antigen PCIA1; DDA1; DDA1_HUMAN; DET1 and DDB1 associated 1; DET1 and DDB1 associated protein 1; DET1- and DDB1-associated protein 1; MGC2594; PCIA-1; PCIA1; Placenta cross immune reaction antigen 1; Placenta cross-immune reaction antigen 1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MADFLKGLPVYNKSNFSRFHADSVCKASNRRPSVYLPTREYPSEQIIVTEKTNILLRYLHQQWDKKNAAKKRDQEQVELEGESSAPPRKVARTDSPDMHEDT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
83
Xenopus
75
Zebrafish
75
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BW61 基板として

Site PTM Type Enzyme
A2 Acetylation
K6 Ubiquitination
K13 Ubiquitination
S23 Phosphorylation
C25 S-Nitrosylation
K26 Ubiquitination
S28 Phosphorylation
S33 Phosphorylation Q96GD4 (AURKB)
Y35 Phosphorylation
T49 Phosphorylation
T52 Phosphorylation
K65 Acetylation
K65 Ubiquitination
K71 Ubiquitination
T93 Phosphorylation
S95 Phosphorylation

研究背景

機能:

May be involved in ubiquitination and subsequent proteasomal degradation of target proteins. Component of the DDD-E2 complexes which may provide a platform for interaction with CUL4A and WD repeat proteins.

サブユニット構造:

Part of the DDD core complex containing DET1, DDA1 and DDB1. The DDD core complex recruits a specific UBE2E enzyme, such as UBE2E1, UBE2E2 UBE2E3, to form specific DDD-E2 complexes.

タンパク質ファミリー:

Belongs to the DDA1 family.

Restrictive clause

 

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