製品: EHD2 Antibody
カタログ: DF12972
タンパク質の説明: Rabbit polyclonal antibody to EHD2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 61 kDa; 61kD(Calculated).
ユニプロット: Q9NZN3
RRID: AB_2845933

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
EHD2 Antibody detects endogenous levels of total EHD2.
RRID:
AB_2845933
引用形式: Affinity Biosciences Cat# DF12972, RRID:AB_2845933.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

EH domain containing 3; EHD2; EHD3; PAST3;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9NZN3 EHD3_HUMAN:

Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta.

タンパク質配列:
MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
83
Xenopus
83
Chicken
83
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NZN3 基板として

Site PTM Type Enzyme
M1 Acetylation
K32 Methylation
Y40 Phosphorylation
T69 Phosphorylation
K124 Ubiquitination
K205 Ubiquitination
K220 Ubiquitination
T226 Phosphorylation
S284 Phosphorylation
K293 Ubiquitination
K299 Ubiquitination
K315 Sumoylation
K316 Ubiquitination
Y339 Phosphorylation
Y453 Phosphorylation
S456 Phosphorylation
T463 Phosphorylation
K475 Ubiquitination
S479 Phosphorylation
K511 Sumoylation

研究背景

機能:

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes. Binding to phosphatidic acid induces its membrane tubulation activity (By similarity). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology. Involved in the recycling of internalized D1 dopamine receptor. Plays a role in cardiac protein trafficking probably implicating ANK2. Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte excitability and cardiac conduction (By similarity). In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function (By similarity). Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with EHD1.

細胞の位置付け:

Recycling endosome membrane>Peripheral membrane protein>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Cilium membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Localizes to the ciliary pocket from where the cilium protrudes (PubMed:25686250). Colocalizes with RAB8A and MYO5B to a cytoplasmic tubular network devoid of RAB11A (By similarity). Colocalizes with ANK2 in myocyte perinuclear region (PubMed:20489164). Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta.

サブユニット構造:

Homooligomer, and heterooligomer with EHD1, EHD2 and EHD4, ATP-binding is required for heterooligomerization. Interacts with PACSIN1 (By similarity). Interacts with PACSIN2 (By similarity). Interacts (via EH domain) with MICALL1. Interacts (via EH domain) with RAB11FIP2. Interacts with ANK2.

タンパク質ファミリー:

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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