EHD2 Antibody - #DF12972
製品: | EHD2 Antibody |
カタログ: | DF12972 |
タンパク質の説明: | Rabbit polyclonal antibody to EHD2 |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 61 kDa; 61kD(Calculated). |
ユニプロット: | Q9NZN3 |
RRID: | AB_2845933 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12972, RRID:AB_2845933.
折りたたみ/展開
EH domain containing 3; EHD2; EHD3; PAST3;
免疫原
Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta.
- Q9NZN3 EHD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NZN3 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
K32 | Methylation | Uniprot | |
Y40 | Phosphorylation | Uniprot | |
T69 | Phosphorylation | Uniprot | |
K124 | Ubiquitination | Uniprot | |
K205 | Ubiquitination | Uniprot | |
K220 | Ubiquitination | Uniprot | |
T226 | Phosphorylation | Uniprot | |
S284 | Phosphorylation | Uniprot | |
K293 | Ubiquitination | Uniprot | |
K299 | Ubiquitination | Uniprot | |
K315 | Sumoylation | Uniprot | |
K316 | Ubiquitination | Uniprot | |
Y339 | Phosphorylation | Uniprot | |
Y453 | Phosphorylation | Uniprot | |
S456 | Phosphorylation | Uniprot | |
T463 | Phosphorylation | Uniprot | |
K475 | Ubiquitination | Uniprot | |
S479 | Phosphorylation | Uniprot | |
K511 | Sumoylation | Uniprot |
研究背景
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes. Binding to phosphatidic acid induces its membrane tubulation activity (By similarity). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology. Involved in the recycling of internalized D1 dopamine receptor. Plays a role in cardiac protein trafficking probably implicating ANK2. Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte excitability and cardiac conduction (By similarity). In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function (By similarity). Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with EHD1.
Recycling endosome membrane>Peripheral membrane protein>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Cilium membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Localizes to the ciliary pocket from where the cilium protrudes (PubMed:25686250). Colocalizes with RAB8A and MYO5B to a cytoplasmic tubular network devoid of RAB11A (By similarity). Colocalizes with ANK2 in myocyte perinuclear region (PubMed:20489164). Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals (By similarity).
Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta.
Homooligomer, and heterooligomer with EHD1, EHD2 and EHD4, ATP-binding is required for heterooligomerization. Interacts with PACSIN1 (By similarity). Interacts with PACSIN2 (By similarity). Interacts (via EH domain) with MICALL1. Interacts (via EH domain) with RAB11FIP2. Interacts with ANK2.
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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