製品: ENOPH1 Antibody
カタログ: DF12977
タンパク質の説明: Rabbit polyclonal antibody to ENOPH1
アプリケーション: WB
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Bovine, Horse, Rabbit, Dog
分子量: 29 kDa; 29kD(Calculated).
ユニプロット: Q9UHY7
RRID: AB_2845938

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ENOPH1 Antibody detects endogenous levels of total ENOPH1.
RRID:
AB_2845938
引用形式: Affinity Biosciences Cat# DF12977, RRID:AB_2845938.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

2 3 diketo 5 methylthio 1 phosphopentane phosphatase; 2; 3-diketo-5-methylthio-1-phosphopentane phosphatase; Acireductone synthase; DKFZp586M0524; E 1 enzyme; E1; Enolase phosphatase 1; Enolase phosphatase E1; Enolase-phosphatase E1; ENOPH_HUMAN; Enoph1; FLJ12594; MASA homolog; MST145; MSTP145 protein;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MVVLSVPAEVTVILLDIEGTTTPIAFVKDILFPYIEENVKEYLQTHWEEEECQQDVSLLRKQAEEDAHLDGAVPIPAASGNGVDDLQQMIQAVVDNVCWQMSLDRKTTALKQLQGHMWRAAFTAGRMKAEFFADVVPAVRKWREAGMKVYIYSSGSVEAQKLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFLTDVTREASAAEEADVHVAVVVRPGNAGLTDDEKTYYSLITSFSELYLPSST

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHY7 基板として

Site PTM Type Enzyme
K111 Ubiquitination
K183 Ubiquitination
K187 Ubiquitination
K195 Ubiquitination
S199 Phosphorylation

研究背景

機能:

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Monomer.

タンパク質ファミリー:

Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.

研究領域

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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