ERO1L Antibody - #DF12984
| 製品: | ERO1L Antibody |
| カタログ: | DF12984 |
| タンパク質の説明: | Rabbit polyclonal antibody to ERO1L |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 54 kDa; 54kD(Calculated). |
| ユニプロット: | Q96HE7 |
| RRID: | AB_2845945 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12984, RRID:AB_2845945.
折りたたみ／展開
Endoplasmic oxidoreductin 1 like protein; Endoplasmic oxidoreductin-1-like protein; Endoplasmic reticulum oxidoreductase 1 alpha; Endoplasmic reticulum oxidoreductin 1-like; ERO1 alpha; ERO1 L; ERO1 Lalpha; ERO1 like protein alpha; ERO1-alpha; ERO1-L; ERO1-L-alpha; ERO1-like (S. cerevisiae); ERO1-like alpha; ERO1-like protein alpha; ERO1-like, S. cerevisiae, homolog of, alpha; ERO1A; ERO1A_HUMAN; ERO1L; ERO1LA; Oxidoreductin 1 Lalpha; Oxidoreductin-1-L-alpha; PRO865; UNQ434;
免疫原
Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
- Q96HE7 ERO1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGRGWGFLFGLLGAVWLLSSGHGEEQPPETAAQRCFCQVSGYLDDCTCDVETIDRFNNYRLFPRLQKLLESDYFRYYKVNLKRPCPFWNDISQCGRRDCAVKPCQSDEVPDGIKSASYKYSEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDNFCEADDIQSPEAEYVDLLLNPERYTGYKGPDAWKIWNVIYEENCFKPQTIKRPLNPLASGQGTSEENTFYSWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQETWLEKKWGHNITEFQQRFDGILTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKMLLLEILHEIKSFPLHFDENSFFAGDKKEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKLIANMPESGPSYEFHLTRQEIVSLFNAFGRISTSVKELENFRNLLQNIH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96HE7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y59 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| R75 | Methylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| C131 | S-Nitrosylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y248 | Phosphorylation | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| N280 | N-Glycosylation | Uniprot | |
| T293 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| Y431 | Phosphorylation | Uniprot |
研究背景
Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.
N-glycosylated.
The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397 (By similarity). The regulatory Cys-99/Cys-104 disulfide bond stabilizes the other regulatory bond Cys-94/Cys-131.
Endoplasmic reticulum membrane>Peripheral membrane protein>Lumenal side.
Note: The association with ERP44 is essential for its retention in the endoplasmic reticulum.
Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
Predominantly monomer. May function both as a monomer and a homodimer. Interacts with PDILT.
Belongs to the EROs family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
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