HERC5 Antibody - #DF13058
| 製品: | HERC5 Antibody |
| カタログ: | DF13058 |
| タンパク質の説明: | Rabbit polyclonal antibody to HERC5 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Bovine, Horse, Sheep |
| 分子量: | 117 kDa; 117kD(Calculated). |
| ユニプロット: | Q9UII4 |
| RRID: | AB_2846019 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13058, RRID:AB_2846019.
折りたたみ／展開
CEB1; CEBP1; Cyclin E binding protein 1; Cyclin-E-binding protein 1; E3 ISG15--protein ligase HERC5; HECT domain and RCC1 like domain containing protein 5; HECT domain and RCC1-like domain-containing protein 5; Hect domain and RLD 5; HECT E3 ubiquitin ligase; HERC5; HERC5_HUMAN; Herc6;
免疫原
Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
- Q9UII4 HERC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERRSRRKSRRNGRSTAGKAAATQPAKSPGAQLWLFPSAAGLHRALLRRVEVTRQLCCSPGRLAVLERGGAGVQVHQLLAGSGGARTPKCIKLGKNMKIHSVDQGAEHMLILSSDGKPFEYDNYSMKHLRFESILQEKKIIQITCGDYHSLALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRDQLMPLPVKVSSSEELKLESHTSEKELIMIAGGNQSILLWIKKENSYVNLKRTIPTLNEGTVKRWIADVETKRWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARNIFKELTQKDWITNMITTCLKDNLLKRLPFHSPPQEALEIFFLLPECPMMHISNNWESLVVPFAKVVCKMSDQSSLVLEEYWATLQESTFSKLVQMFKTAVICQLDYWDESAEENGNVQALLEMLKKLHRVNQVKCQLPESIFQVDELLHRLNFFVEVCRRYLWKMTVDASENVQCCVIFSHFPFIFNNLSKIKLLHTDTLLKIESKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPEYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHWDRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKMCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UII4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K19 | Ubiquitination | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K541 | Sumoylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| Y661 | Phosphorylation | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| K807 | Ubiquitination | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| K867 | Ubiquitination | Uniprot | |
| Y868 | Phosphorylation | Uniprot | |
| Y871 | Phosphorylation | Uniprot | |
| K890 | Ubiquitination | Uniprot | |
| K898 | Ubiquitination | Uniprot | |
| K906 | Ubiquitination | Uniprot | |
| K918 | Ubiquitination | Uniprot | |
| K946 | Ubiquitination | Uniprot |
研究背景
Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
ISGylated.
Cytoplasm>Perinuclear region.
Note: Associated with the polyribosomes, probably via the 60S subunit.
Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
Binds to CCNA1, CCNB1, CCND1 and CCNE1. Interacts with UBE2L6. Interacts with IRF3, this interaction is marginal in resting cells but enhanced upon viral infection. Interacts with influenza A virus NS1.
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