KLF6 Antibody - #DF13114

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製品: KLF6 Antibody
カタログ: DF13114
タンパク質の説明: Rabbit polyclonal antibody to KLF6
アプリケーション: WB
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 43 kDa; 32kD(Calculated).
ユニプロット: Q99612
RRID: AB_2846074

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(80%), Bovine(80%), Sheep(80%), Rabbit(92%), Dog(80%)
クローナリティ:
Polyclonal
特異性:
KLF6 Antibody detects endogenous levels of total KLF6.
RRID:
AB_2846074
引用形式: Affinity Biosciences Cat# DF13114, RRID:AB_2846074.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

免疫原

免疫原:

A synthesized peptide derived from human KLF6, corresponding to a region within the internal amino acids.

Uniprot:

Q99612(KLF6_HUMAN) >>Visit HPA database.

遺伝子(ID):
KLF6(1316)
発現特異性:
Q99612 KLF6_HUMAN:

Highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. Weakly expressed in pancreas, lung, liver, heart and skeletal muscle. Also expressed in fetal brain, spleen and thymus.

タンパク質配列:
MDVLPMCSIFQELQIVHETGYFSALPSLEEYWQQTCLELERYLQSEPCYVSASEIKFDSQEDLWTKIILAREKKEESELKISSSPPEDTLISPSFCYNLETNSLNSDVSSESSDSSEELSPTAKFTSDPIGEVLVSSGKLSSSVTSTPPSSPELSREPSQLWGCVPGELPSPGKVRSGTSGKPGDKGNGDASPDGRRRVHRCHFNGCRKVYTKSSHLKAHQRTHTGEKPYRCSWEGCEWRFARSDELTRHFRKHTGAKPFKCSHCDRCFSRSDHLALHMKRHL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Pig
80
Bovine
80
Sheep
80
Dog
80
Xenopus
73
Chicken
73
Horse
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Transcriptional activator (By similarity). Binds a GC box motif. Could play a role in B-cell growth and development.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. Weakly expressed in pancreas, lung, liver, heart and skeletal muscle. Also expressed in fetal brain, spleen and thymus.

タンパク質ファミリー:

The acidic N-terminal part may favor interaction with the basic domain of transcription factors.

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF6, the motif is inactive.

Belongs to the krueppel C2H2-type zinc-finger protein family.

参考文献

1). miRNA‑21 promotes the progression of acute liver failure via the KLF6/autophagy/IL‑23 signaling pathway. Molecular medicine reports, 2024 (PubMed: 38516774) [IF=3.4]
2). miR‑181a‑5p regulates the proliferation and apoptosis of glomerular mesangial cells by targeting KLF6. Experimental and Therapeutic Medicine, 2020 (PubMed: 32742352) [IF=2.4]

Application: WB    Species: human    Sample: glomerular mesangial cell

Figure 7.| miR‑181a‑5p may regulate human glomerular mesangial cell proliferation and apoptosis via the Wnt/β‑catenin signaling pathway. (A) The protein expression levels of the associated factors in different groups were assessed by western blot analysis and (B) the results quantified. **P<0.01, *P<0.05. miR,microRNA; KLF6, kruppel-like factor 6; cells were treated with miR mimics control NC, negative control (cells were treated with miR mimics control).
3). FebuxostatAlleviatesAllergicRhinitis by Inhibiting Inflammation and Monocyte Adhesion in Human Nasal Epithelial Cells via Regulating KLF6. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 36118091)

Application: WB    Species: Human    Sample: hNECs

Figure 2 The expression level of chemokines, adhesion molecules, and KLF6 expression in histamine-treated hNECs were repressed by febuxostat. (a). The expression level of chemokines was measured by the RT-qPCR assay, (b) the production of chemokines was evaluated by the ELISA assay, (c) the expression level of CAMs was checked by the RT-qPCR assay, (d) the expression level of CAMs was measured by the Western blotting assay (p < 0.05 and p < 0.01), (e) the adhesion between hNECs and U937 monocytes was inhibited by febuxostat. The attached U937 monocytes were detected using the calcein-AM staining assay (p < 0.05 and p < 0.01), (f) the expression level of KLF6 was evaluated by the RT-qPCR assay, and (g) the expression level of KLF6 was determined by the Western blotting assay (p < 0.05 and p < 0.01). Immunofluorescence magnification was 10×.

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