MARS Antibody - #DF13138
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13138, RRID:AB_2846098.
折りたたみ／展開
cytoplasmic; FLJ35667; Mars; Methionine tRNA ligase 1; Methionine tRNA ligase 1 cytoplasmic; Methionine tRNA ligase; Methionine tRNA synthetase; Methionine--tRNA ligase; Methionyl tRNA synthetase cytoplasmic; Methionyl-tRNA synthetase; MetRS; MRS; MTRNS; SYMC_HUMAN;
免疫原
- P56192 SYMC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLFVSDGVPGCLPVLAAAGRARGRAEVLISTVGPEDCVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGSVRRALTHIDHSLSRQNCPFLAGETESLADIVLWGALYPLLQDPAYLPEELSALHSWFQTLSTQEPCQRAAETVLKQQGVLALRPYLQKQPQPSPAEGRAVTNEPEEEELATLSEEEIAMAVTAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKALEEGLTPQEICDKYHIIHADIYRWFNISFDIFGRTTTPQQTKITQDIFQQLLKRGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P56192 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Q9P2K8 (EIF2AK4) | Uniprot |
| K335 | Ubiquitination | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| Y344 | Phosphorylation | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K375 | Acetylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| R376 | Methylation | Uniprot | |
| S472 | Phosphorylation | Q9P2K8 (EIF2AK4) | Uniprot |
| T482 | Phosphorylation | Uniprot | |
| K500 | Ubiquitination | Uniprot | |
| Y549 | Phosphorylation | Uniprot | |
| S644 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | Q9P2K8 (EIF2AK4) | Uniprot |
| K663 | Ubiquitination | Uniprot | |
| T675 | Phosphorylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| K726 | Acetylation | Uniprot | |
| K726 | Ubiquitination | Uniprot | |
| K729 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| T824 | Phosphorylation | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| T833 | Phosphorylation | Uniprot | |
| K838 | Ubiquitination | Uniprot | |
| K875 | Ubiquitination | Uniprot | |
| K881 | Ubiquitination | Uniprot |
研究背景
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Plays a role in the synthesis of ribosomal RNA in the nucleolus.
Cytoplasm>Cytosol. Nucleus>Nucleolus.
Note: Localizes to the nucleolus in proliferative cells but disappears in quiescent cells.
Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 Ref.24,. Forms a linear complex that contains MARS1, EEF1E1, EPRS1 and AIMP2 that is at the core of the multisubunit complex.
Belongs to the class-I aminoacyl-tRNA synthetase family.
研究領域
· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.
· Metabolism > Metabolism of other amino acids > Selenocompound metabolism.
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