MLN64 Antibody - #DF13153
| 製品: | MLN64 Antibody |
| カタログ: | DF13153 |
| タンパク質の説明: | Rabbit polyclonal antibody to MLN64 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 50 kDa; 51kD(Calculated). |
| ユニプロット: | Q14849 |
| RRID: | AB_2846113 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13153, RRID:AB_2846113.
折りたたみ／展開
CAB 1; CAB1; Es 64; Es64; FLJ41370; Metastatic lymph node gene 64 protein; Metastatic lymph node protein 64; MLN 64; Mln64; Protein CAB 1; Protein CAB1; Protein MLN 64; Protein MLN64; StAR related lipid transfer protein 3; StAR-related lipid transfer protein 3; STAR3_HUMAN; STARD 3; StARD3; START domain containing 3; START domain-containing protein 3; Steroidogenic acute regulatory protein related;
免疫原
- Q14849 STAR3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKLPRELTRDLERSLPAVASLGSSLSHSQSLSSHLLPPPEKRRAISDVRRTFCLFVTFDLLFISLLWIIELNTNTGIRKNLEQEIIQYNFKTSFFDIFVLAFFRFSGLLLGYAVLRLRHWWVIAVTTLVSSAFLIVKVILSELLSKGAFGYLLPIVSFVLAWLETWFLDFKVLPQEAEEERWYLAAQVAVARGPLLFSGALSEGQFYSPPESFAGSDNESDEEVAGKKSFSAQEREYIRQGKEATAVVDQILAQEENWKFEKNNEYGDTVYTIEVPFHGKTFILKTFLPCPAELVYQEVILQPERMVLWNKTVTACQILQRVEDNTLISYDVSAGAAGGVVSPRDFVNVRRIERRRDRYLSSGIATSHSAKPPTHKYVRGENGPGGFIVLKSASNPRVCTFVWILNTDLKGRLPRYLIHQSLAATMFEFAFHLRQRISELGARA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14849 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot |
研究背景
Sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes. Creates contact site between the endoplasmic reticulum and late endosomes: localizes to late endosome membranes and contacts the endoplasmic reticulum via interaction with VAPA and VAPB. Acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes. May also mediate cholesterol transport between other membranes, such as mitochondria membrane or cell membrane. However, such results need additional experimental evidences; probably mainly mediates cholesterol transport from the endoplasmic reticulum to endosomes. Does not activate transcriptional cholesterol sensing. Able to bind other lipids, such as lutein, a xanthophyll carotenoids that form the macular pigment of the retina.
Late endosome membrane>Multi-pass membrane protein.
Note: Localizes to contact sites between the endoplasmic reticulum and late endosomes: associates with the endoplasmic reticulum membrane via interaction with VAPA and VAPB (PubMed:24105263).
Expressed in retina.
Homodimer. Interacts (via the MENTAL domain) with STARD3NL. Interacts (via FFAT motif) with VAPA. Interacts (via FFAT motif) with VAPB.
The FFAT motif mediates interaction with VAPA and VAPB.
The START domain mediates lipid-transfer between membranes. It contains a hydrophobic cavity able to accommodate one lipid molecule, thereby serving as a 'hydrophobic bridge' across the aqueous gap between donor and acceptor organelle membranes.
The MENTAL domain anchors the protein in endosome membranes and exposes the START domain in the cytosol (PubMed:11053434). It binds cholesterol and mediates homotypic as well as heterotypic interactions between STARD3 and STARD3NL (PubMed:15718238, PubMed:16709157).
Belongs to the STARD3 family.
研究領域
· Organismal Systems > Digestive system > Cholesterol metabolism.
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