NIT2 Antibody - #DF13186
| 製品: | NIT2 Antibody |
| カタログ: | DF13186 |
| タンパク質の説明: | Rabbit polyclonal antibody to NIT2 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 30 kDa; 31kD(Calculated). |
| ユニプロット: | Q9NQR4 |
| RRID: | AB_2846146 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13186, RRID:AB_2846146.
折りたたみ／展開
CUA002; MGC111199; NIT 2; Nit protein 2; NIT2; NIT2_HUMAN; Nitrilase family member 2; Nitrilase homolog 2; Omega amidase NIT2; Omega-amidase NIT2;
免疫原
Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.
- Q9NQR4 NIT2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIPGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAVEMKKP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NQR4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S26 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K68 | Acetylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| Y145 | Phosphorylation | Uniprot | |
| C146 | S-Nitrosylation | Uniprot | |
| Y165 | Phosphorylation | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| T237 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| K249 | Acetylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K250 | Acetylation | Uniprot | |
| R265 | Methylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| Y269 | Phosphorylation | Uniprot |
研究背景
Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
Cytoplasm.
Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.
Homodimer.
Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
研究領域
· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.
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