NUDT16 Antibody - #DF13198
| 製品: | NUDT16 Antibody |
| カタログ: | DF13198 |
| タンパク質の説明: | Rabbit polyclonal antibody to NUDT16 |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep |
| 分子量: | 21 kDa; 21kD(Calculated). |
| ユニプロット: | Q96DE0 |
| RRID: | AB_2846158 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13198, RRID:AB_2846158.
折りたたみ／展開
FLJ31265; FLJ34034; FLJ36248; Nucleoside diphosphate-linked moiety X motif 16; NUD16_HUMAN; Nudix (nucleoside diphosphate linked moiety X) type motif 16; Nudix motif 16; NUDT 16; NUDT16; U8 snoRNA-binding protein H29K; U8 snoRNA-decapping enzyme;
免疫原
- Q96DE0 NUD16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGARRLELGEALALGSGWRHACHALLYAPDPGMLFGRIPLRYAILMQMRFDGRLGFPGGFVDTQDRSLEDGLNRELREELGEAAAAFRVERTDYRSSHVGSGPRVVAHFYAKRLTLEELLAVEAGATRAKDHGLEVLGLVRVPLYTLRDGVGGLPTFLENSFIGSAREQLLEALQDLGLLQSGSISGLKIPAHH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96DE0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y111 | Phosphorylation | Uniprot | |
| T116 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot |
研究背景
RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms. The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP. Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
Nucleus. Nucleus>Nucleoplasm. Nucleus>Nucleolus. Cytoplasm.
Note: Localized predominantly in the cytoplasm (PubMed:21070968). Localized in nucleolus, and in a minor proportion in distinct foci in the nucleoplasm (By similarity).
Expressed strongly in lung, kidney, adrenal gland, testis, heart and brain.
Homodimer.
Belongs to the Nudix hydrolase family. NUDT16 subfamily.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
· Metabolism > Nucleotide metabolism > Purine metabolism.
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