Phospho-CPI17alpha (Thr38) Antibody - #AF3473

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製品: Phospho-CPI17alpha (Thr38) Antibody
カタログ: AF3473
タンパク質の説明: Rabbit polyclonal antibody to Phospho-CPI17alpha (Thr38)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit
分子量: 22kDa; 17kD(Calculated).
ユニプロット: Q96A00
RRID: AB_2834911

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説明書
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プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-CPI17alpha (Thr38) Antibody detects endogenous levels of CPI17alpha only when phosphorylated at Threonine 38.
RRID:
AB_2834911
引用形式: Affinity Biosciences Cat# AF3473, RRID:AB_2834911.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17kDa PKC potentiated inhibitory protein of PP1; CPI 17 alpha; CPI 17; CPI 17alpha; CPI-17; CPI17; PKC potentiated inhibitory protein of PP1; PKC potentiated inhibitory protein of PP1, 17-KD; PP14A_HUMAN; PPP1INL; Ppp1r14a; Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD; Protein kinase C-potentiated inhibitor protein of 17 kDa; Protein phosphatase 1; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A;

免疫原

免疫原:
Uniprot:

Q96A00(PP14A_HUMAN) >>Visit HPA database.

遺伝子(ID):
PPP1R14A(94274)
発現特異性:
Q96A00 PP14A_HUMAN:

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

タンパク質の説明:
PPP1R14A a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity.
タンパク質配列:
MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
73
Horse
0
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96A00 基板として

Site PTM Type Enzyme
R5 Methylation
S12 Phosphorylation O43293 (DAPK3)
S16 Phosphorylation
R19 Methylation
R21 Methylation
S26 Phosphorylation
K32 Methylation
T38 Phosphorylation Q05655 (PRKCD) , P17612 (PRKACA) , O75116 (ROCK2) , Q02156 (PRKCE) , O43293 (DAPK3) , Q15139 (PRKD1) , Q13976 (PRKG1) , P17252 (PRKCA) , Q13418 (ILK) , Q05513 (PRKCZ) , Q13153 (PAK1) , P98161 (PKD1)
K40 Methylation
S128 Phosphorylation
S130 Phosphorylation Q9UQM7 (CAMK2A)
S134 Phosphorylation
S136 Phosphorylation

研究背景

機能:

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

タンパク質ファミリー:

Belongs to the PP1 inhibitor family.

研究領域

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

参考文献

1). Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm-Induced Sudden Cardiac Death. International journal of molecular sciences, 2024 (PubMed: 38474189) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 3 Effects of CAS on the expression of phosphorylated CPI-17 in rat coronary arteries. (A,B) Representative photomicrographs and semi-quantitative data of p-CPI-17 protein levels detected via IHC staining. Lower enlarged images are from upper images. n = 6 per group. Scale bar: 100 µm (upper images) or 50 µm (lower images). (C) Representative immunofluorescent images of p-CPI-17 (red) and DAPI (blue) in the coronary arteries of rats. Scale bar: 50 µm. (D) The mean fluorescence intensity of p-CPI-17 per section was quantified. n = 6 per group. (E,F) Western blot and quantitative analysis of p-CPI-17 protein levels of rat coronary arteries. n = 6 per group. Data presented as mean ± SEM. ** p < 0.01 vs. control group. CPI-17: C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa; p-CPI-17: phosphorylated C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa.

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