Cleaved-Caspase 7 (Asp198) Antibody - #AF4023

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製品: Cleaved-Caspase 7 (Asp198) Antibody
カタログ: AF4023
タンパク質の説明: Rabbit polyclonal antibody to Cleaved-Caspase 7 (Asp198)
アプリケーション: WB
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 20kDa, 25 kDa; 34kD(Calculated).
ユニプロット: P55210
RRID: AB_2846219

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(86%)
クローナリティ:
Polyclonal
特異性:
Cleaved-Caspase 7 (Asp198) Antibody detects endogenous levels of fragment of activated Caspase 7 resulting from cleavage adjacent to Asp198.
RRID:
AB_2846219
引用形式: Affinity Biosciences Cat# AF4023, RRID:AB_2846219.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Apoptotic protease Mch-3; Apoptotic protease MCH3; CASP-7; CASP7; CASP7_HUMAN; Caspase 7; Caspase 7 apoptosis related cysteine peptidase; Caspase-7 subunit p11; Caspase7; CMH 1; CMH-1; CMH1; ICE LAP3; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; MCH3;

免疫原

免疫原:
Uniprot:

P55210(CASP7_HUMAN) >>Visit HPA database.

遺伝子(ID):
CASP7(840)
発現特異性:
P55210 CASP7_HUMAN:

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

タンパク質配列:
MADDQGCIEEQGVEDSANEDSVDAKPDRSSFVPSLFSKKKKNVTMRSIKTTRDRVPTYQYNMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEALFKCFRSLGFDVIVYNDCSCAKMQDLLKKASEEDHTNAACFACILLSHGEENVIYGKDGVTPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDTDANPRYKIPVEADFLFAYSTVPGYYSWRSPGRGSWFVQALCSILEEHGKDLEIMQILTRVNDRVARHFESQSDDPHFHEKKQIPCVVSMLTKELYFSQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
86
Zebrafish
67
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55210 基板として

Site PTM Type Enzyme
A2 Acetylation
S16 Phosphorylation
S29 Phosphorylation
S30 Phosphorylation Q13177 (PAK2)
S34 Phosphorylation
S37 Phosphorylation
K38 Methylation
K38 Ubiquitination
T44 Phosphorylation
S47 Phosphorylation
K69 Ubiquitination
K80 Ubiquitination
K92 Ubiquitination
K99 Ubiquitination
K153 Ubiquitination
T157 Phosphorylation
K160 Ubiquitination
K172 Ubiquitination
T173 Phosphorylation Q13177 (PAK2)
K177 Ubiquitination
S234 Phosphorylation
S239 Phosphorylation Q13177 (PAK2)

研究背景

機能:

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.

PTMs:

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

サブユニット構造:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Interacts with ATXN3 (short isoform 1).

タンパク質ファミリー:

Belongs to the peptidase C14A family.

研究領域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

参考文献

1). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH, 2020 (PubMed: 32710621) [IF=14.9]

Application: WB    Species: mouse    Sample: liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.
2). Excessive mechanical stress induces chondrocyte apoptosis through TRPV4 in an anterior cruciate ligament-transected rat osteoarthritis model. LIFE SCIENCES, 2019 (PubMed: 31055086) [IF=6.1]

Application: WB    Species: rat    Sample: chondrocytes

Fig. 5.| Excessive mechanical stress stimulated caspase-8-dependent apoptotic signaling pathway in chondrocytes by activating TRPV4. (A) TRPV4, calmodulin,FADD, cleaved caspase-8, cleaved caspase-3, cleaved caspase-6, and cleaved caspase-7 protein expression was detected by western blotting.
3). TMEM97 is transcriptionally activated by YY1 and promotes colorectal cancer progression via the GSK-3β/β-catenin signaling pathway. Human Cell, 2022 (PubMed: 35907137) [IF=4.3]
4). Lappaconitine sulfate inhibits proliferation and induces mitochondrial-mediated apoptosis via regulating PI3K/AKT/GSK3β signaling pathway in HeLa cells. Naunyn-Schmiedeberg's Archives of Pharmacology, 2023 (PubMed: 37306713) [IF=3.6]
5). Costunolide attenuates oxygen‑glucose deprivation/reperfusion‑induced mitochondrial‑mediated apoptosis in PC12 cells. Molecular Medicine Reports, 2021 (PubMed: 33786628) [IF=3.4]

Application: WB    Species: Rat    Sample: PC12 cells

Figure 8. Effects of CT on the expression of Apaf-1, Bcl-2, Bax, cleaved-Caspase-9, cleaved-Caspase-7, cleaved-Caspase-3 in PC12 cells following OGD/R. The stripe diagram represents Western blot analysis of Apaf-1, Bcl-2, Bax, cleaved-Caspase-9, cleaved-Caspase-7, cleaved-Caspase-3, procaspase-9, procaspase-7 and procaspase-3. α-tubulin was usedd as the loading control. The bar chart represents the quantitative analysis of Apaf-1, Bcl-2, Bax, cleaved-Caspase-9/procaspase-9, cleaved-Caspase-7/procaspase-7 and cleaved-Caspase-3/procaspase-3 expression. Values are presented as the mean ± standard deviation (n=3). #P<0.05, compared with the control group; *P<0.05, compared with OGD/R group. CT, costunolide; OGD/R, oxygen-glucose deprivation/reperfusion.
6). Lappaconitine Sulfate Inhibits Proliferation and Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells through the Reactive Oxygen Species-Dependent Mitochondrial Pathway. PHARMACOLOGY, 2020 (PubMed: 32062649) [IF=3.1]

Application: WB    Species: Human    Sample: HepG2 cells

Fig. 2. LS induced apoptosis of HepG2 cells. a, b Apoptosis was analyzed by flow cytometry after treatment with LS for 48 h. c, d The apoptosis-related proteins were determined by western blotting after treatment with LS for 24 h. Cells were pretreated with 60 μmol/L z-VAD-fmk for 1.5 h and then incubated with 400 μg/mL LS for 48 and 24 h, respectively, (e) the cell viability was examined by CCK-8 assay and (f, g) the proteins were examined by western blotting. Data are expressed as mean ± SD (n = 3). * p < 0.05, ** p < 0.01 compared with control group. ++ p < 0.01 represent the significant difference between HepG2 and HUVEC cells at uniform concentration. ## p < 0.01 represent the significant difference between LS and LS + z-VAD-fmk. LS, lappaconitine sulfate.

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