Neurofilament heavy polypeptide Antibody - #DF13211
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13211, RRID:AB_2846230.
折りたたみ／展開
200 kDa neurofilament protein; CMT2CC; Nefh; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; NF H; NF-H; NFH; NFH_HUMAN;
免疫原
- P12036 NFH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGEEEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPPEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSPAEAKSPVKEEAKSPAEVKSPEKAKSPTKEEAKSPEKAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLDVKSPEAKTPAKEEARSPADKFPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPKKAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDKTLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P12036 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K31 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| Y385 | Phosphorylation | Uniprot | |
| R412 | Methylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| T501 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S511 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S526 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K531 | Ubiquitination | Uniprot | |
| S532 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S540 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| S574 | Phosphorylation | Uniprot | |
| S580 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| S634 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| S654 | Phosphorylation | Uniprot | |
| K657 | Acetylation | Uniprot | |
| K659 | Acetylation | Uniprot | |
| K659 | Ubiquitination | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| K663 | Acetylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | Uniprot | |
| S710 | Phosphorylation | Uniprot | |
| S716 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| S730 | Phosphorylation | Uniprot | |
| T738 | Phosphorylation | Uniprot | |
| S744 | Phosphorylation | Uniprot | |
| S752 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| S793 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| S807 | Phosphorylation | Uniprot | |
| S828 | Phosphorylation | Uniprot | |
| K895 | Ubiquitination | Uniprot | |
| K907 | Acetylation | Uniprot | |
| K908 | Acetylation | Uniprot | |
| T911 | Phosphorylation | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| S993 | Phosphorylation | Uniprot |
研究背景
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Cytoplasm.
Belongs to the intermediate filament family.
研究領域
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
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