Nav1.5 Antibody - #DF13217
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13217, RRID:AB_2846236.
折りたたみ／展開
Cardiac tetrodotoxin insensitive voltage dependent sodium channel alpha subunit; CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1; ICCD; IVF; LQT3; PFHB1; Scn5a (gene name); Scn5a; SCN5A_HUMAN; Sodium channel protein cardiac muscle alpha subunit; Sodium channel protein cardiac muscle subunit alpha; Sodium channel protein type 5 subunit alpha; Sodium channel protein type V alpha subunit; Sodium channel protein type V subunit alpha; SSS1; VF1; Voltage gated sodium channel alpha subunit Nav1.5; Voltage-gated sodium channel subunit alpha Nav1.5;
免疫原
Found in jejunal circular smooth muscle cells (at protein level). Expressed in human atrial and ventricular cardiac muscle but not in adult skeletal muscle, brain, myometrium, liver, or spleen. Isoform 4 is expressed in brain.
- Q14524 SCN5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSTTLQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVNSHERRSKRRKRMSSGTEECGEDRLPKSDSEDGPRAMNHLSLTRGLSRTSMKPRSSRGSIFTFRRRDLGSEADFADDENSTAGESESHHTSLLVPWPLRRTSAQGQPSPGTSAPGHALHGKKNSTVDCNGVVSLLGAGDPEATSPGSHLLRPVMLEHPPDTTTPSEEPGGPQMLTSQAPCVDGFEEPGARQRALSAVSVLTSALEELEESRHKCPPCWNRLAQRYLIWECCPLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYNMTSEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRDSDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDREMNNLQLALARIQRGLRFVKRTTWDFCCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEEGEQPGQGTPGDPEPVCVPIAVAESDTDDQEEDEENSLGTEEESSKQQESQPVSGGPEAPPDSRTWSQVSATASSEAEASASQADWRQQWKAEPQAPGCGETPEDSCSEGSTADMTNTAELLEQIPDLGQDVKDPEDCFTEGCVRRCPCCAVDTTQAPGKVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSQCESLNLTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKINILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPTLPNSNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYSVLSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSAMVIQRAFRRHLLQRSLKHASFLFRQQAGSGLSEEDAPEREGLIAYVMSENFSRPLGPPSSSSISSTSFPPSYDSVTRATSDNLQVRGSDYSHSEDLADFPPSPDRDRESIV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14524 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| K343 | Acetylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| T486 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| R513 | Methylation | Uniprot | |
| S516 | Phosphorylation | Q13557 (CAMK2D) | Uniprot |
| S519 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| R526 | Methylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| T531 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| T570 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S577 | Phosphorylation | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| T594 | Phosphorylation | Q13557 (CAMK2D) | Uniprot |
| S664 | Phosphorylation | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| R680 | Methylation | Uniprot | |
| Y694 | Phosphorylation | Uniprot | |
| S1003 | Phosphorylation | Uniprot | |
| T1094 | Phosphorylation | Uniprot | |
| S1103 | Phosphorylation | Uniprot | |
| S1104 | Phosphorylation | Uniprot | |
| K1479 | Acetylation | Uniprot | |
| K1493 | Acetylation | Uniprot | |
| Y1494 | Phosphorylation | P06241 (FYN) | Uniprot |
| Y1495 | Phosphorylation | P06241 (FYN) | Uniprot |
| S1503 | Phosphorylation | Uniprot | |
| S1865 | Phosphorylation | Uniprot | |
| S1885 | Phosphorylation | Uniprot | |
| T1895 | Phosphorylation | Uniprot | |
| S1920 | Phosphorylation | Uniprot | |
| S1925 | Phosphorylation | Uniprot | |
| S1934 | Phosphorylation | Uniprot | |
| S1937 | Phosphorylation | Uniprot | |
| S1985 | Phosphorylation | Uniprot | |
| S1998 | Phosphorylation | Uniprot | |
| S2007 | Phosphorylation | Uniprot |
研究背景
This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant Na(+) channel isoform. This channel is responsible for the initial upstroke of the action potential. Channel inactivation is regulated by intracellular calcium levels.
Ubiquitinated by NEDD4L; which promotes its endocytosis. Does not seem to be ubiquitinated by NEDD4 or WWP2.
Phosphorylation at Ser-1503 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents (Probable). Regulated through phosphorylation by CaMK2D (By similarity).
Lacks the cysteine which covalently binds the conotoxin GVIIJ. This cysteine (position 868) is speculated in other sodium channel subunits alpha to be implied in covalent binding with the sodium channel subunit beta-2 or beta-4.
Cell membrane>Multi-pass membrane protein. Cytoplasm>Perinuclear region. Cell membrane>Sarcolemma>T-tubule.
Note: RANGRF promotes trafficking to the cell membrane.
Found in jejunal circular smooth muscle cells (at protein level). Expressed in human atrial and ventricular cardiac muscle but not in adult skeletal muscle, brain, myometrium, liver, or spleen. Isoform 4 is expressed in brain.
Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2 (By similarity). Interacts with NEDD4, NEDD4L, WWP2 and GPD1L. Interacts with CALM. Interacts with FGF13; the interaction is direct and FGF13 may regulate SNC5A density at membranes and function. May also interact with FGF12 and FGF14. Interacts with the spider Jingzhaotoxin-I (AC P83974, AC B1P1B7, AC B1P1B8). Interacts with ANK3.
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
The IQ domain mediates association with calmodulin.
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.5/SCN5A subfamily.
研究領域
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
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