SC35 Antibody - #DF13219
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13219, RRID:AB_2846238.
折りたたみ／展開
35 kDa; arginine/serine-rich 2; PR264; Protein PR264; SC 35; SC-35; SC35; Serine/arginine-rich splicing factor 2; SFRS 2; SFRS2; SFRS2A; Splicing component 35 kDa; Splicing component; Splicing factor; Splicing factor arginine/serine rich 2; Splicing factor SC35; Splicing speckle; Splicing speckles; SR splicing factor 2; SRp30b; SRSF2; SRSF2_HUMAN;
免疫原
- Q01130 SRSF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRSPRRRRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPVSKRESKSRSRSKSPPKSPEEEGAVSS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q01130 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| R5 | Methylation | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| Y23 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| T29 | Phosphorylation | Uniprot | |
| R31 | Methylation | Uniprot | |
| K36 | Acetylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| R39 | Methylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| R55 | Methylation | Uniprot | |
| R66 | Methylation | Uniprot | |
| R83 | Methylation | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| R94 | Methylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| R103 | Methylation | Uniprot | |
| R109 | Methylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S128 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S130 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S142 | Phosphorylation | Uniprot | |
| Y144 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K172 | Methylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot |
研究背景
Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
Extensively phosphorylated on serine residues in the RS domain. Phosphorylated by SRPK2 and this causes its redistribution from the nuclear speckle to nucleoplasm and controls cell fate decision in response to cisplatin treatment. KAT5/TIP60 inhibits its phosphorylation by preventing SRPK2 nuclear translocation.
Acetylation on Lys-52 by KAT5/TIP60 promotes its proteasomal degradation. This effect is counterbalanced by HDAC6, which positively controls SRSF2 protein level by deacetylating it and preventing its proteasomal degradation.
Nucleus. Nucleus>Nucleoplasm. Nucleus speckle.
Note: Phosphorylation by SRPK2 provokes its redistribution from the nuclear specke to nucleoplasm.
Interacts with BRDT (By similarity). In vitro, self-associates and binds SRSF1/SFRS1 (ASF/SF2), SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with CCNL1 and CCNL2. Interacts with SCAF11. Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus).
Belongs to the splicing factor SR family.
研究領域
· Genetic Information Processing > Transcription > Spliceosome.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
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