Citrate synthetase Antibody - #DF13222

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製品: Citrate synthetase Antibody
カタログ: DF13222
タンパク質の説明: Rabbit polyclonal antibody to Citrate synthetase
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 51kDa; 52kD(Calculated).
ユニプロット: O75390
RRID: AB_2846241

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Citrate synthetase Antibody detects endogenous levels of total Citrate synthetase.
RRID:
AB_2846241
引用形式: Affinity Biosciences Cat# DF13222, RRID:AB_2846241.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CISY_HUMAN; Citrate synthase; Citrate synthase, mitochondrial; citrate synthetase; Cs; EC 2.3.3; EC 2.3.3.1;

免疫原

免疫原:
Uniprot:

O75390(CISY_HUMAN) >>Visit HPA database.

遺伝子(ID):
CS(1431)
タンパク質配列:
MALLTAAARLLGTKNASCLVLAARHASASSTNLKDILADLIPKEQARIKTFRQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDPDEGIRFRGFSIPECQKLLPKAKGGEEPLPEGLFWLLVTGHIPTEEQVSWLSKEWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAVTALNSESNFARAYAQGISRTKYWELIYEDSMDLIAKLPCVAAKIYRNLYREGSGIGAIDSNLDWSHNFTNMLGYTDHQFTELTRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPMFKLVAQLYKIVPNVLLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTEGLMKFVDSKSG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75390 基板として

Site PTM Type Enzyme
T5 Phosphorylation
T13 Phosphorylation
K43 Acetylation
K43 Ubiquitination
K76 Acetylation
K76 Ubiquitination
Y80 Phosphorylation
T82 Phosphorylation
S83 Phosphorylation
S97 Phosphorylation
C101 S-Nitrosylation
K103 Acetylation
K103 Ubiquitination
S190 Phosphorylation
K193 Acetylation
C211 S-Nitrosylation
K215 Acetylation
K215 Ubiquitination
K321 Acetylation
K327 Acetylation
K327 Ubiquitination
Y331 Phosphorylation
S338 Phosphorylation
K366 Acetylation
K366 Ubiquitination
K375 Acetylation
Y381 Phosphorylation
K382 Acetylation
K382 Ubiquitination
K393 Acetylation
K393 Ubiquitination
K395 Methylation
K450 Acetylation
S451 Phosphorylation
K459 Acetylation
K459 Ubiquitination
S463 Phosphorylation

研究背景

PTMs:

Methylated. Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity.

細胞の位置付け:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer.

タンパク質ファミリー:

Belongs to the citrate synthase family.

研究領域

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

参考文献

1). A novel HSF1 activator ameliorates non‐alcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation. BRITISH JOURNAL OF PHARMACOLOGY, 2022 (PubMed: 34783017) [IF=7.3]

Application: WB    Species: Human    Sample: LX-2 cells

FIGURE 4 SYSU-3d promotes lipid oxidation by activating the heat shock factor 1 (HSF1)/ PPARγ coactivator-1α (PGC-1ɑ) metabolic pathway (a–e) HepG-2 cells were exposed to palmitic acid (PA; 0.75 mM) in the presence or absence of SYSU-3d (1 μM) for 24 h and then analysed. (a) mtDNA/nDNA. (b) Determination of the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) from metabolic flux analyses. (c) Free fatty acid (FFA) uptake assay using the probe TF2-C12. (d) ROS level determination by flow cytometry. (e) Expression of citrate synthase (CS), malate dehydrogenase 2 (MDH2) and mitochondrial complexes; protein levels were quantified and normalized to Actin. (f–h) Determination of CS, mitochondrial complex I and II activity by normalizing to protein level. (i–k) Determination of oxygen consumption rate (OCR) ability, mitochondrial copy numbers (indicated by Mito-tracker probe) and ROS level (indicated by DCFH-DA probe) in HSF1−/− cells. (l and m) Determination of triglyceride (TG) levels, cell survival, mitochondrial copy numbers and ROS levels in PGC-1ɑ−/− cells. (n) Determination of OCR in PGC-1ɑ−/− cells. (o) Expression of metabolic regulators and inflammation- and apoptosis-related proteins in PGC-1ɑ−/− cells; protein levels were quantified and normalized to Actin; n = 5 independent experiments. *P

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