Clathrin heavy chain Antibody - #DF13223
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13223, RRID:AB_2846242.
免疫原
Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.
- P53675 CLH2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQILPVRFQEHFQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVTIIDMSDPMAPIRRPISAESAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMAEEVIFWKWVSVNTVALVTETAVYHWSMEGDSQPMKMFDRHTSLVGCQVIHYRTDEYQKWLLLVGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFAEFKMEGNAKPATLFCFAVRNPTGGKLHIIEVGQPAAGNQPFVKKAVDVFFPPEAQNDFPVAMQIGAKHGVIYLITKYGYLHLYDLESGVCICMNRISADTIFVTAPHKPTSGIIGVNKKGQVLSVCVEEDNIVNYATNVLQNPDLGLRLAVRSNLAGAEKLFVRKFNTLFAQGSYAEAAKVAASAPKGILRTRETVQKFQSIPAQSGQASPLLQYFGILLDQGQLNKLESLELCHLVLQQGRKQLLEKWLKEDKLECSEELGDLVKTTDPMLALSVYLRANVPSKVIQCFAETGQFQKIVLYAKKVGYTPDWIFLLRGVMKISPEQGLQFSRMLVQDEEPLANISQIVDIFMENSLIQQCTSFLLDALKNNRPAEGLLQTWLLEMNLVHAPQVADAILGNKMFTHYDRAHIAQLCEKAGLLQQALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSVECLHAMLSANIRQNLQLCVQVASKYHEQLGTQALVELFESFKSYKGLFYFLGSIVNFSQDPDVHLKYIQAACKTGQIKEVERICRESSCYNPERVKNFLKEAKLTDQLPLIIVCDRFGFVHDLVLYLYRNNLQRYIEIYVQKVNPSRTPAVIGGLLDVDCSEEVIKHLIMAVRGQFSTDELVAEVEKRNRLKLLLPWLESQIQEGCEEPATHNALAKIYIDSNNSPECFLRENAYYDSSVVGRYCEKRDPHLACVAYERGQCDLELIKVCNENSLFKSEARYLVCRKDPELWAHVLEETNPSRRQLIDQVVQTALSETRDPEEISVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYISRLDNYDALDIASIAVSSALYEEAFTVFHKFDMNASAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLAQAQLQKDLVKEAINSYIRGDDPSSYLEVVQSASRSNNWEDLVKFLQMARKKGRESYIETELIFALAKTSRVSELEDFINGPNNAHIQQVGDRCYEEGMYEAAKLLYSNVSNFARLASTLVHLGEYQAAVDNSRKASSTRTWKEVCFACMDGQEFRFAQLCGLHIVIHADELEELMCYYQDRGYFEELILLLEAALGLERAHMGMFTELAILYSKFKPQKMLEHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGQFKDIITKVANVELCYRALQFYLDYKPLLINDLLLVLSPRLDHTWTVSFFSKAGQLPLVKPYLRSVQSHNNKSVNEALNHLLTEEEDYQGLRASIDAYDNFDNISLAQQLEKHQLMEFRCIAAYLYKGNNWWAQSVELCKKDHLYKDAMQHAAESRDAELAQKLLQWFLEEGKRECFAACLFTCYDLLRPDMVLELAWRHNLVDLAMPYFIQVMREYLSKVDKLDALESLRKQEEHVTEPAPLVFDFDGHE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P53675 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K78 | Ubiquitination | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| T214 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K450 | Acetylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| K456 | Acetylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| K506 | Acetylation | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| Y510 | Phosphorylation | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K742 | Acetylation | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| Y754 | Phosphorylation | Uniprot | |
| K764 | Acetylation | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| Y799 | Phosphorylation | Uniprot | |
| Y803 | Phosphorylation | Uniprot | |
| K806 | Ubiquitination | Uniprot | |
| S841 | Phosphorylation | Uniprot | |
| T842 | Phosphorylation | Uniprot | |
| K851 | Ubiquitination | Uniprot | |
| K856 | Ubiquitination | Uniprot | |
| S889 | Phosphorylation | Uniprot | |
| K911 | Ubiquitination | Uniprot | |
| K932 | Ubiquitination | Uniprot | |
| K941 | Ubiquitination | Uniprot | |
| K951 | Ubiquitination | Uniprot | |
| T963 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| S989 | Phosphorylation | Uniprot | |
| S1016 | Phosphorylation | Uniprot | |
| S1019 | Phosphorylation | Uniprot | |
| K1034 | Ubiquitination | Uniprot | |
| T1038 | Phosphorylation | Uniprot | |
| Y1043 | Phosphorylation | Uniprot | |
| S1045 | Phosphorylation | Uniprot | |
| Y1096 | Phosphorylation | Uniprot | |
| K1122 | Ubiquitination | Uniprot | |
| Y1168 | Phosphorylation | Uniprot | |
| T1171 | Phosphorylation | Uniprot | |
| S1181 | Phosphorylation | Uniprot | |
| T1318 | Phosphorylation | Uniprot | |
| S1341 | Phosphorylation | Uniprot | |
| K1347 | Ubiquitination | Uniprot | |
| K1387 | Ubiquitination | Uniprot | |
| K1392 | Ubiquitination | Uniprot | |
| T1396 | Phosphorylation | Uniprot | |
| K1449 | Acetylation | Uniprot | |
| K1449 | Ubiquitination | Uniprot | |
| K1461 | Ubiquitination | Uniprot | |
| S1462 | Phosphorylation | Uniprot | |
| K1529 | Ubiquitination | Uniprot | |
| K1535 | Ubiquitination | Uniprot | |
| K1612 | Ubiquitination | Uniprot | |
| K1621 | Ubiquitination | Uniprot |
研究背景
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity).
Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side.
Note: Cytoplasmic face of coated pits and vesicles.
Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.
Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium (By similarity). May interact with OCRL (By similarity).
The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.
The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It constitutes a major protein-protein interaction node (By similarity).
Belongs to the clathrin heavy chain family.
研究領域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
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