TRPA1 Antibody - #DF13269

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製品: TRPA1 Antibody
カタログ: DF13269
タンパク質の説明: Rabbit polyclonal antibody to TRPA1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 100~150kD; 128kD(Calculated).
ユニプロット: O75762
RRID: AB_2846288

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MS Report
HPLC Report
MSDS
説明書
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IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(86%)
クローナリティ:
Polyclonal
特異性:
TRPA1 Antibody detects endogenous levels of total TRPA1.
RRID:
AB_2846288
引用形式: Affinity Biosciences Cat# DF13269, RRID:AB_2846288.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ANKTM 1; ANKTM1; Ankyrin-like with transmembrane domains protein 1; Transformation sensitive protein p120; Transformation-sensitive protein p120; Transient receptor potential cation channel subfamily A member 1; TRPA 1; Trpa1; TRPA1_HUMAN;

免疫原

免疫原:
Uniprot:

O75762(TRPA1_HUMAN) >>Visit HPA database.

遺伝子(ID):
TRPA1(8989)
発現特異性:
O75762 TRPA1_HUMAN:

Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells (PubMed:10066796).

タンパク質配列:
MKRSLRKMWRPGEKKEPQGVVYEDVPDDTEDFKESLKVVFEGSAYGLQNFNKQKKLKRCDDMDTFFLHYAAAEGQIELMEKITRDSSLEVLHEMDDYGNTPLHCAVEKNQIESVKFLLSRGANPNLRNFNMMAPLHIAVQGMNNEVMKVLLEHRTIDVNLEGENGNTAVIIACTTNNSEALQILLKKGAKPCKSNKWGCFPIHQAAFSGSKECMEIILRFGEEHGYSRQLHINFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGSVDIVNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASWNIVNLLLSKGAQVDIKDNFGRNFLHLTVQQPYGLKNLRPEFMQMQQIKELVMDEDNDGCTPLHYACRQGGPGSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQQASFLHLALHNKRKEVVLTIIRSKRWDECLKIFSHNSPGNKCPITEMIEYLPECMKVLLDFCMLHSTEDKSCRDYYIEYNFKYLQCPLEFTKKTPTQDVIYEPLTALNAMVQNNRIELLNHPVCKEYLLMKWLAYGFRAHMMNLGSYCLGLIPMTILVVNIKPGMAFNSTGIINETSDHSEILDTTNSYLIKTCMILVFLSSIFGYCKEAGQIFQQKRNYFMDISNVLEWIIYTTGIIFVLPLFVEIPAHLQWQCGAIAVYFYWMNFLLYLQRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDPFSSPLLSIIQTFSMMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTSLEKKLPLWFLRKVDQKSTIVYPNKPRSGGMLFHIFCFLFCTGEIRQEIPNADKSLEMEILKQKYRLKDLTFLLEKQHELIKLIIQKMEIISETEDDDSHCSFQDRFKKEQMEQRNSRWNTVLRAVKAKTHHLEP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
86
Xenopus
86
Chicken
86
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75762 基板として

Site PTM Type Enzyme
S43 Phosphorylation
S86 Phosphorylation
S113 Phosphorylation
T304 Phosphorylation
T305 Phosphorylation
S317 Phosphorylation
K337 Methylation
S354 Phosphorylation
S363 Phosphorylation
K403 Acetylation
S428 Phosphorylation
S438 Phosphorylation
S448 Phosphorylation
S470 Phosphorylation
K503 Methylation
K504 Methylation
T673 Phosphorylation
T764 Phosphorylation
S972 Phosphorylation

研究背景

機能:

Receptor-activated non-selective cation channel involved in pain detection and possibly also in cold perception, oxygen concentration perception, cough, itch, and inner ear function. Shows 8-fold preference for divalent over monovalent cations. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of irritants, such as allylthiocyanate (AITC) from mustard oil or wasabi, cinnamaldehyde, diallyl disulfide (DADS) from garlic, and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as an ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system (By similarity).

PTMs:

TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain.

Hydroxylation is required for TRPA1 activity inhibition in normoxia. In hypoxia, the decrease in oxygene concentration diminishes the activity of the hydroxylase EGLN1, thus relieving TRPA1 from inhibition and ultimately leading to channel activation.

Oxidation of Cys-633 and Cys-856 in hyperoxia may override the hydroxylase EGLN1-mediated inhibition, causing TRPA1 activation.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells.

サブユニット構造:

Homotetramer. Interacts with TMEM100 (By similarity). Interacts with EGLN1 (By similarity). Interacts with the scorpion wasabi receptor toxin at the same site that electrophiles but in a non-covalent manner.

タンパク質ファミリー:

C-terminal helices from the four subunits associate to form atypical coiled coil structure; this region is probably involved in binding the inositol polyphosphates that are required for optimal channel activity (in vitro).

The ANK repeat domain consists of a convex stem structure formed by five ANK repeats and 11 additional ANK repeats that form a crescent-shaped structure that surrounds the protein core.

Belongs to the transient receptor (TC 1.A.4) family.

研究領域

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

参考文献

1). Discovery of PRDM16-Mediated TRPA1 Induction as the Mechanism for Low Tubulo-Interstitial Fibrosis in Diabetic Kidney Disease. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38072665) [IF=15.1]

Application: WB    Species: Mouse    Sample:

Figure 3 PRDM16 interacts with the promoter of TRPA1 to transactivate its expression. HA‐PRDM16‐RFP stable BUMPT cell line was treated with DOX, and then subjected to NG or HG treatment for 48 h to perform the analysis of ChIP‐seq using HA antibody. A) ChIP‐seq results showing that total 13 462 and 16 415 genes were upregulated and downregulated respectively in HG versus NG group. B) ChIP‐seq image visualized in 10 bp resolution in IGV Genome Browser. C) ChIP assays showing the binding of PRDM16 to the P4 (500‐0 bp) region of TRPA1 gene promoter. D) Map of the promoter region of TRPA1. E) Luciferase reporter assay showing PRDM16 may activate P4‐1 and P4‐2 but no other TRPA1 promoter fragments. F) RT‐qPCR analysis showing HG induction of TRPA1 mRNA. G) Immunoblots showing HG induction of TRPA1 protein. H) Densitometry analysis of immunoblot bands. I–K) Suppression of TRPA1 induction by PRDM16 knockdown in HG‐treated cells. BUMPT cells were transfected with PRDM16 shRNA and then subjected to HG or NG incubation. RT–qPCR and Immunoblot analyses show that knockdown of PRDM16 suppressed the expression of TRPA1. I) RT‐qPCR analysis of TRPA1 mRNA. J) Immunoblot analysis of TRPA1 protein. K) Densitometry analysis of immunoblot bands. L–N) Increased TRPA1 induction by PRDM16 in HG‐treated cells. We established stable HA‐PRDM16‐RFP‐transfected BUMPT cells that overexpress PRDM16 upon doxycycline (DOX) induction. RT–qPCR analysis and Immunoblot results indicates that overexpression of PRDM16 increased the expression of TRPA1.Data are expressed as mean ± SD (n = 6). # P < 0.05, versus scrambled oligos with NG group. * P < 0.05, versus scrambled oligos with HG group.
2). Protein phosphatase 2Cm-regulated branched-chain amino acid catabolic defect in dorsal root ganglion neurons drives pain sensitization. Acta Neuropathologica Communications, 2024 [IF=6.2]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 4 Inhibiting the CCL5/CCR5 signaling pathway attenuates hyperalgesia in DRG PP2Cm-deficient mice a, Quantification for release of CCL5 in the L4-L5 DRGs of PP2Cm-ctrl and PP2Cm-cKO mice, as detected by ELISA. n = 4 mice/group. b, Illustrative cartoon depicting the intrathecal administration of an anti-CCL5 neutralizing antibody. c, Quantification for release of CCL5 in the L4-L5 DRGs of PP2Cm-cKO + IgG, PP2Cm-cKO + anti-CCL5 1d and PP2Cm-cKO + anti-CCL5 3d mice. n = 4 mice/group. d-f, Mechanical withdrawal thresholds (d), withdrawal latencies to heat (e) and cold stimulus (f) on day 0 (baseline, BL), 1, and 3 post-injection in PP2Cm-cKO mice treated with IgG or anti-CCL5 neutralizing antibody. n = 5–6 mice/group. g, The L4-L5 DRGs from PP2Cm-ctrl and PP2Cm-cKO mice were double stained with PP2Cm (green) and CCR5 (red). The corner image in white square is the zoomed-in image of the area in the smaller white square. Scale bar: 20 μm. h, i, Fluorescent quantifications for PP2Cm (h) and CCR5 (i) in DRG sections from PP2Cm-ctrl and PP2Cm-cKO mice. n = 3 sections from 3 mice/group. j, A representative cartoon illustrating the intrathecal injection of CCR5 antagonist maraviroc or Ccr5 small interfering RNA (siCcr5). k-m, Mechanical withdrawal thresholds (k), withdrawal latencies to heat (l) and cold stimulus (m) at BL, 1 h, and 24 h post-injection in PP2Cm-cKO mice treated with Vehicle or maraviroc. n = 6 mice/group. n, The mRNA levels of Ccr5 in the L4-L5 DRGs from PP2Cm-cKO + siCtrl, PP2Cm-cKO + siCcr5 2d, and PP2Cm-cKO + siCcr5 8d mice (to β-actin). n = 3 experimental repeats (6 mice)/group. o-q, Mechanical withdrawal thresholds (o), withdrawal latencies to heat (p) and cold stimulus (q) at BL, 2d, and 8d post-injection in PP2Cm-cKO mice treated with siCtrl or siCcr5. n = 5–6 mice/group. Data are shown as the mean ± SEM. Statistical tests used were unpaired two-tailed Student’s t-test (a, h, i), one-way ANOVA with Tukey’s multiple comparisons test (c, n), and two-way repeated-measures ANOVA with Bonferroni’s post hoc test (d-f, k-m, o-q). *p 
3). Neochlorogenic acid anchors MCU-based calcium overload for cancer therapy. Food & Function, 2021 (PubMed: 34672304) [IF=6.1]
4). Kemin capsule ameliorates post-infectious cough by modulating the PI3K/AKT signaling pathway and TRPA1/TRPV1 channels. JOURNAL OF ETHNOPHARMACOLOGY, 2024 [IF=4.8]
5). Chrysin mitigated neuropathic pain and peripheral sensitization in knee osteoarthritis rats by repressing the RAGE/PI3K/AKT pathway regulated by HMGB1. Cytokine, 2024 (PubMed: 38749277) [IF=3.8]
6). Fluorescence visualization of the neuropathic pain triad in trigeminal neuralgia. Journal of Biophotonics, 2023 (PubMed: 36369929) [IF=2.8]
7). TRPA1 involved in miR-141-5p-alleviated neuropathic pain induced by oxaliplatin. NEUROREPORT, 2021 (PubMed: 33534373) [IF=1.7]

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