PU.1/Spi1 Antibody - #DF13270

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製品: PU.1/Spi1 Antibody
カタログ: DF13270
タンパク質の説明: Rabbit polyclonal antibody to PU.1/Spi1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 31kDa; 31kD(Calculated).
ユニプロット: P17947
RRID: AB_2846289

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
PU.1/Spi1 Antibody detects endogenous levels of total PU.1/Spi1.
RRID:
AB_2846289
引用形式: Affinity Biosciences Cat# DF13270, RRID:AB_2846289.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

;transcription factor spi1; 31 kDa Transforming Protein; 31 kDa-transforming protein; cb1086; Hematopoietic transcription factor PU.1; OF; oncogene spi1; PU.1; SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of; SFPI1; si:by184l24.2; SPI 1; SPI 1 proto oncogene; SPI A; Spi1; SPI1_HUMAN; Spleen focus forming virus (SFFV) proviral integration oncogene spi1; Spleen focus forming virus proviral integration oncogene spi1; Transcription factor PU.1;

免疫原

免疫原:
Uniprot:

P17947(SPI1_HUMAN) >>Visit HPA database.

遺伝子(ID):
SPI1(6688)
タンパク質配列:
MLQACKMEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEGERQSPPLEVSDGEADGLEPGPGLLPGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
71
Rabbit
57
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17947 基板として

Site PTM Type Enzyme
S17 Phosphorylation
S140 Phosphorylation
S146 Phosphorylation
S253 Phosphorylation

研究背景

機能:

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Binds DNA as a monomer. Interacts with CEBPD and NONO (By similarity). Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Interacts with CEBPE.

タンパク質ファミリー:

Belongs to the ETS family.

研究領域

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

参考文献

1). Benefits of Valsartan and Amlodipine in Lipolysis through PU.1 Inhibition in Fructose-Induced Adiposity. Nutrients, 2022 (PubMed: 36145135) [IF=5.9]

Application: WB    Species: Mouse    Sample:

Figure 2 Effects of different fructose concentrations on (A) ATF3 and PU.1 protein levels in adipocytes in vitro, (B) ATF3, PU.1, MGL, ATGL, and SCD1 mRNA levels in adipocytes in vitro, and (C) the immunohistochemistry intensity of ATF3 and PU.1 obtained from our previous study [17]. N = 6, the results from 6 independent experiments in cell culture experiments and from 6 mice in tissue used study. Values are presented as mean ± SD. *, **, and *** denote p < 0.05,

Application: IHC    Species: Mouse    Sample:

Figure 2 Effects of different fructose concentrations on (A) ATF3 and PU.1 protein levels in adipocytes in vitro, (B) ATF3, PU.1, MGL, ATGL, and SCD1 mRNA levels in adipocytes in vitro, and (C) the immunohistochemistry intensity of ATF3 and PU.1 obtained from our previous study [17]. N = 6, the results from 6 independent experiments in cell culture experiments and from 6 mice in tissue used study. Values are presented as mean ± SD. *, **, and *** denote p < 0.05,

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