CHD4 Antibody - #DF13295

(8)  
製品: CHD4 Antibody
カタログ: DF13295
タンパク質の説明: Rabbit polyclonal antibody to CHD4
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 218kDa; 218kD(Calculated).
ユニプロット: Q14839
RRID: AB_2846314

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
CHD4 Antibody detects endogenous levels of total CHD4.
RRID:
AB_2846314
引用形式: Affinity Biosciences Cat# DF13295, RRID:AB_2846314.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ATP dependent helicase CHD4; ATP-dependent helicase CHD4; CHD 4; CHD-4; CHD4; CHD4_HUMAN; Chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi-2 autoantigen 218 kDa protein; Mi2 beta; Mi2-beta;

免疫原

免疫原:
Uniprot:

Q14839(CHD4_HUMAN) >>Visit HPA database.

遺伝子(ID):
CHD4(1108)
タンパク質配列:
MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14839 基板として

Site PTM Type Enzyme
S15 Phosphorylation
S27 Phosphorylation
S44 Phosphorylation
T46 Phosphorylation
S85 Phosphorylation
S86 Phosphorylation
S103 Phosphorylation
S105 Phosphorylation
S108 Phosphorylation
Y110 Phosphorylation
K115 Acetylation
K116 Acetylation
K118 Acetylation
K119 Acetylation
K123 Acetylation
S150 Phosphorylation
S151 Phosphorylation
S168 Phosphorylation
K179 Ubiquitination
K195 Ubiquitination
K273 Acetylation
K275 Acetylation
S277 Phosphorylation
K285 Acetylation
K297 Ubiquitination
S303 Phosphorylation
K304 Acetylation
K304 Ubiquitination
S308 Phosphorylation
S309 Phosphorylation
S310 Phosphorylation
S319 Phosphorylation
S325 Phosphorylation
T360 Phosphorylation
Y365 Phosphorylation
T367 Phosphorylation
Y372 Phosphorylation
S428 Phosphorylation
K499 Ubiquitination
K504 Ubiquitination
S515 Phosphorylation
T517 Phosphorylation
T529 Phosphorylation P24941 (CDK2)
S531 Phosphorylation P24941 (CDK2)
K533 Ubiquitination
S585 Phosphorylation
K594 Ubiquitination
K604 Ubiquitination
K618 Sumoylation
K618 Ubiquitination
S631 Phosphorylation
K634 Ubiquitination
K635 Ubiquitination
K668 Ubiquitination
R679 Methylation
K696 Ubiquitination
T703 Phosphorylation
T705 Phosphorylation
T709 Phosphorylation
K711 Sumoylation
K711 Ubiquitination
K832 Ubiquitination
K884 Acetylation
K884 Ubiquitination
K959 Ubiquitination
K964 Ubiquitination
S979 Phosphorylation
K987 Acetylation
K987 Ubiquitination
R1000 Methylation
S1007 Phosphorylation
K1016 Acetylation
K1016 Ubiquitination
K1017 Acetylation
K1017 Ubiquitination
K1051 Ubiquitination
K1057 Ubiquitination
S1073 Phosphorylation
K1092 Ubiquitination
K1206 Ubiquitination
T1207 Phosphorylation
S1209 Phosphorylation
K1212 Ubiquitination
K1220 Ubiquitination
K1228 Sumoylation
K1228 Ubiquitination
S1244 Phosphorylation
S1245 Phosphorylation
K1252 Ubiquitination
T1265 Phosphorylation
Y1276 Phosphorylation
K1281 Ubiquitination
R1288 Methylation
K1304 Sumoylation
K1304 Ubiquitination
S1308 Phosphorylation
Y1313 Phosphorylation
K1316 Ubiquitination
R1319 Methylation
K1335 Ubiquitination
S1349 Phosphorylation Q13315 (ATM)
S1370 Phosphorylation
K1390 Ubiquitination
K1396 Ubiquitination
K1398 Sumoylation
K1398 Ubiquitination
K1422 Ubiquitination
S1500 Phosphorylation
K1505 Ubiquitination
K1528 Ubiquitination
K1529 Sumoylation
S1531 Phosphorylation
S1535 Phosphorylation
S1537 Phosphorylation
T1540 Phosphorylation
T1542 Phosphorylation
S1544 Phosphorylation
T1545 Phosphorylation
T1549 Phosphorylation
T1553 Phosphorylation
K1565 Sumoylation
S1570 Phosphorylation
K1572 Sumoylation
K1572 Ubiquitination
S1576 Phosphorylation
K1584 Ubiquitination
C1594 S-Nitrosylation
T1595 Phosphorylation
S1602 Phosphorylation
K1643 Acetylation
K1643 Ubiquitination
K1647 Sumoylation
K1647 Ubiquitination
S1648 Phosphorylation
T1653 Phosphorylation
K1660 Ubiquitination
K1670 Sumoylation
T1679 Phosphorylation
T1722 Phosphorylation
K1724 Ubiquitination
T1725 Phosphorylation
K1766 Ubiquitination
Y1804 Phosphorylation
S1808 Phosphorylation
C1827 S-Nitrosylation
S1831 Phosphorylation
K1837 Ubiquitination
S1839 Phosphorylation
K1844 Ubiquitination
K1852 Ubiquitination
K1855 Ubiquitination
K1865 Ubiquitination
S1888 Phosphorylation
T1904 Phosphorylation

研究背景

機能:

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

細胞の位置付け:

Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Associates with centrosomes in interphase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with KLF1; the interaction depends on sumoylation of KLF1, and leads to its transcriptional repression (By similarity). Interacts directly with IKFZ1 in the NuRD complex. Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. Part of a complex containing ATR and HDAC2. Interacts with TRIM27. Interacts with ZGPAT; the interaction is direct. Interacts with BCL6. Interacts with BRD4. Interacts with PCNT. Interacts with SETX. Interacts with HDAC1.

タンパク質ファミリー:

Belongs to the SNF2/RAD54 helicase family.

研究領域

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.