VGLUT2 Antibody - #DF13296

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製品: VGLUT2 Antibody
カタログ: DF13296
タンパク質の説明: Rabbit polyclonal antibody to VGLUT2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 64kDa; 64kD(Calculated).
ユニプロット: Q9P2U8
RRID: AB_2846315

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
VGLUT2 Antibody detects endogenous levels of total VGLUT2.
RRID:
AB_2846315
引用形式: Affinity Biosciences Cat# DF13296, RRID:AB_2846315.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Differentiation associated BNPI; Differentiation associated Na dependent inorganic phosphate cotransporter; Differentiation associated Na(+) dependent inorganic phosphate cotransporter; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; DNPI; SLC17A6; Sodium dependent inorganic phosphate cotransporter; Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6; Solute carrier family 17 member 6; Vesicular glutamate transporter 2; Vesicular glutamate transporter type 2; VGLU2_HUMAN; VGLUT 2; VGluT2;

免疫原

免疫原:
Uniprot:

Q9P2U8(VGLU2_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC17A6(57084)
発現特異性:
Q9P2U8 VGLU2_HUMAN:

Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.

タンパク質配列:
MESVKQRILAPGKEGLKNFAGKSLGQIYRVLEKKQDTGETIELTEDGKPLEVPERKAPLCDCTCFGLPRRYIIAIMSGLGFCISFGIRCNLGVAIVDMVNNSTIHRGGKVIKEKAKFNWDPETVGMIHGSFFWGYIITQIPGGYIASRLAANRVFGAAILLTSTLNMLIPSAARVHYGCVIFVRILQGLVEGVTYPACHGIWSKWAPPLERSRLATTSFCGSYAGAVIAMPLAGILVQYTGWSSVFYVYGSFGMVWYMFWLLVSYESPAKHPTITDEERRYIEESIGESANLLGAMEKFKTPWRKFFTSMPVYAIIVANFCRSWTFYLLLISQPAYFEEVFGFEISKVGMLSAVPHLVMTIIVPIGGQIADFLRSKQILSTTTVRKIMNCGGFGMEATLLLVVGYSHTRGVAISFLVLAVGFSGFAISGFNVNHLDIAPRYASILMGISNGVGTLSGMVCPIIVGAMTKNKSREEWQYVFLIAALVHYGGVIFYAIFASGEKQPWADPEETSEEKCGFIHEDELDEETGDITQNYINYGTTKSYGATTQANGGWPSGWEKKEEFVQGEVQDSHSYKDRVDYS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P2U8 基板として

Site PTM Type Enzyme
Y28 Phosphorylation
K109 Ubiquitination
S171 Phosphorylation
Y177 Phosphorylation
Y281 Phosphorylation

研究背景

機能:

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

細胞の位置付け:

Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane. Membrane>Multi-pass membrane protein. Cell junction>Synapse>Synaptosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.

タンパク質ファミリー:

Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

研究領域

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

参考文献

1). Complement C1q-mediated microglial synaptic elimination by enhancing desialylation underlies sevoflurane-induced developmental neurotoxicity. Cell & bioscience, 2024 (PubMed: 38556890) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 1 Cognitive dysfunction and synapse loss in the hippocampus of mice after neonatal sevoflurane exposures. A Scheme of neonatal mice receiving repeated sevoflurane and the MWM test. Neonatal mice were exposed to 3% sevoflurane on PNDs 6, 8, and 10, and then the memory and learning abilities were assessed using the MWM test on PNDs 31–36. B Tracking plots of mice. C Sevoflurane reduced the escape latency compared to the control group on PNDs 33–35, as well as the platform crossing times and times spent in the target quadrant. Swimming speed was similar between groups. n = 8. Unpaired t-test and two-way ANOVA. D Representative confocal microscopy images displaying the immunoreactivity of the presynaptic marker Vglut2 (green) and postsynaptic marker PSD95 (red) in the hippocampus. Scale bar = 5 μm. E The sevoflurane group showed decreased density of Vglut2 and PSD95. n = 6. Unpaired t-test. F Colocalization analysis showed that the density of synapses was lower in sevoflurane-treated mice. n = 6. Unpaired t-test. G Representative Western blot bands of Vglut2 and PSD95 in the two groups. (H) Quantification of Western blot showed that the expression of Vglut2 and PSD95 was decreased in sevoflurane-treated mice. n = 4. Unpaired t-test. I Representative Golgi-Cox staining images of dendritic spines in the hippocampus. Scale bar = 5 μm. J Quantification of the density of dendritic spines showed that the sevoflurane group had a lower spine density. n = 6. Unpaired t-test. Data are mean ± SEM. *P 

Application: IF/ICC    Species: Mouse    Sample:

Fig. 1 Cognitive dysfunction and synapse loss in the hippocampus of mice after neonatal sevoflurane exposures. A Scheme of neonatal mice receiving repeated sevoflurane and the MWM test. Neonatal mice were exposed to 3% sevoflurane on PNDs 6, 8, and 10, and then the memory and learning abilities were assessed using the MWM test on PNDs 31–36. B Tracking plots of mice. C Sevoflurane reduced the escape latency compared to the control group on PNDs 33–35, as well as the platform crossing times and times spent in the target quadrant. Swimming speed was similar between groups. n = 8. Unpaired t-test and two-way ANOVA. D Representative confocal microscopy images displaying the immunoreactivity of the presynaptic marker Vglut2 (green) and postsynaptic marker PSD95 (red) in the hippocampus. Scale bar = 5 μm. E The sevoflurane group showed decreased density of Vglut2 and PSD95. n = 6. Unpaired t-test. F Colocalization analysis showed that the density of synapses was lower in sevoflurane-treated mice. n = 6. Unpaired t-test. G Representative Western blot bands of Vglut2 and PSD95 in the two groups. (H) Quantification of Western blot showed that the expression of Vglut2 and PSD95 was decreased in sevoflurane-treated mice. n = 4. Unpaired t-test. I Representative Golgi-Cox staining images of dendritic spines in the hippocampus. Scale bar = 5 μm. J Quantification of the density of dendritic spines showed that the sevoflurane group had a lower spine density. n = 6. Unpaired t-test. Data are mean ± SEM. *P 
2). A glutamatergic pathway between the medial habenula and the rostral ventrolateral medulla may regulate cardiovascular function in a rat model of post-traumatic stress disorder. , 2023

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