FKBP51 Antibody - #DF13305

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製品: FKBP51 Antibody
カタログ: DF13305
タンパク質の説明: Rabbit polyclonal antibody to FKBP51
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 51kDa; 51kD(Calculated).
ユニプロット: Q13451
RRID: AB_2846324

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
FKBP51 Antibody detects endogenous levels of total FKBP51.
RRID:
AB_2846324
引用形式: Affinity Biosciences Cat# DF13305, RRID:AB_2846324.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

51 kDa FK506 binding protein 5; 51 kDa FK506 binding protein; 51 kDa FK506-binding protein; 51 kDa FKBP; 54 kDa progesterone receptor associated immunophilin; 54 kDa progesterone receptor-associated immunophilin; AIG 6; AIG6; Androgen regulated protein 6; Androgen-regulated protein 6; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; FKBP 5; FKBP 51; FKBP 54; FKBP-5; FKBP-51; FKBP5; FKBP5_HUMAN; FKBP54; HSP90 binding immunophilin; HSP90-binding immunophilin; MGC111006; OTTHUMP00000016268; P54; Peptidyl prolyl cis trans isomerase; Peptidyl-prolyl cis-trans isomerase FKBP5; Peptidylprolyl cis trans isomerase; PPIase; PPIase FKBP5; Ptg 10; Ptg10; Rotamase; T cell FK506 binding protein;

免疫原

免疫原:

A synthesized peptide derived from human FKBP51, corresponding to a region within the internal amino acids.

Uniprot:

Q13451(FKBP5_HUMAN) >>Visit HPA database.

遺伝子(ID):
FKBP5(2289)
発現特異性:
Q13451 FKBP5_HUMAN:

Widely expressed, enriched in testis compared to other tissues.

タンパク質配列:
MTTDEGAKNNEESPTATVAEQGEDITSKKDRGVLKIVKRVGNGEETPMIGDKVYVHYKGKLSNGKKFDSSHDRNEPFVFSLGKGQVIKAWDIGVATMKKGEICHLLCKPEYAYGSAGSLPKIPSNATLFFEIELLDFKGEDLFEDGGIIRRTKRKGEGYSNPNEGATVEIHLEGRCGGRMFDCRDVAFTVGEGEDHDIPIGIDKALEKMQREEQCILYLGPRYGFGEAGKPKFGIEPNAELIYEVTLKSFEKAKESWEMDTKEKLEQAAIVKEKGTVYFKGGKYMQAVIQYGKIVSWLEMEYGLSEKESKASESFLLAAFLNLAMCYLKLREYTKAVECCDKALGLDSANEKGLYRRGEAQLLMNEFESAKGDFEKVLEVNPQNKAARLQISMCQKKAKEHNERDRRIYANMFKKFAEQDAKEEANKAMGKKTSEGVTNEKGTDSQAMEEEKPEGHV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded. Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1.

PTMs:

Acetylation impairs ability to promote interaction between Akt/AKT1 and PHLPP1. Deacetylation by SIRT7 promotes interaction between Akt/AKT1 and PHLPP1, leading to suppress Akt/AKT1 activation.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed, enriched in testis compared to other tissues.

研究領域

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

参考文献

1). Rosmarinic acid alleviate CORT-induced depressive-like behavior by promoting neurogenesis and regulating BDNF/TrkB/PI3K signaling axis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38070249) [IF=6.9]

Application: WB    Species: Mouse    Sample:

Fig. 3. Effect of RA on the HPA axis and hippocampus region glucocorticoid-related proteins expression in chronic CORT injection depression model mice. (A) The levels of CORT in the serum (n = 6). (B) The levels of ACTH in the serum (n = 6). (C) The levels of CRH in the serum (n = 6). (D) Representative protein bands of glucocorticoid pathway (FKBP51, SGK-1, p-GR, GR, HSP90) (n = 3) in the hippocampus of mice. Statistical graphs of relative protein expression of FKBP51 (E), ratio of p-GR(Ser211)/GR (F), relative protein expression of SGK-1 (G) and relative protein expression of HSP90 (H). Data were normalized to the control and presented as means ± SEM.
2). Novel immunosuppressive effect of FK506 by upregulation of PD‐L1 via FKBP51 in heart transplantation. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2022 (PubMed: 35801698) [IF=4.1]

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