Phospho-HDAC6 (Ser22) Antibody - #AF3485
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(6)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3485, RRID:AB_2834923.
折りたたみ／展開
CPBHM; FLJ16239; HD 6; HD6; HDAC 6; HDAC6; HDAC6_HUMAN; Histone deacetylase 6 (HD6); Histone deacetylase 6; JM 21; JM21; KIAA0901; OTTHUMP00000032398; OTTHUMP00000197663; PPP1R90; Protein phosphatase 1 regulatory subunit 90;
免疫原
- Q9UBN7 HDAC6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDEQLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKEELMLVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASGSVLRLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQQKHRIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKASNWSTTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLVLVAAGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLRALAEGVSASLHTLLGDPCPMLESPGAPCRSAQASVSCALEALEPFWEVLVRSTETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNMMNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRLVEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGHALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKVEDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGEESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTIQENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAYVHHQALLDVKNIAHQNKFGEDMPHPH
PTMs - Q9UBN7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S28 | Phosphorylation | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K36 | Acetylation | Uniprot | |
| K37 | Acetylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y570 | Phosphorylation | P00533 (EGFR) | Uniprot |
| S846 | Phosphorylation | Uniprot | |
| S847 | Phosphorylation | Uniprot | |
| K849 | Acetylation | Uniprot | |
| K849 | Ubiquitination | Uniprot | |
| K853 | Acetylation | Uniprot | |
| K854 | Acetylation | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| K860 | Ubiquitination | Uniprot | |
| T868 | Phosphorylation | Uniprot | |
| K873 | Acetylation | Uniprot | |
| K881 | Acetylation | Uniprot | |
| T923 | Phosphorylation | Uniprot | |
| S925 | Phosphorylation | Uniprot | |
| T993 | Phosphorylation | Uniprot | |
| S995 | Phosphorylation | Uniprot | |
| T1003 | Phosphorylation | Uniprot | |
| T1016 | Phosphorylation | Uniprot | |
| T1021 | Phosphorylation | Uniprot | |
| T1027 | Phosphorylation | Uniprot | |
| T1031 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| T1034 | Phosphorylation | Uniprot | |
| S1035 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| T1040 | Phosphorylation | Uniprot | |
| T1041 | Phosphorylation | Uniprot | |
| T1048 | Phosphorylation | Uniprot | |
| T1055 | Phosphorylation | Uniprot | |
| S1062 | Phosphorylation | Uniprot | |
| K1206 | Ubiquitination | Uniprot |
研究背景
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. In addition to histones, deacetylates other proteins: plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy.
Phosphorylated by AURKA.
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus. Perikaryon. Cell projection>Dendrite. Cell projection>Axon.
Note: It is mainly cytoplasmic, where it is associated with microtubules.
Interacts with SIRT2 (via both phosphorylated, unphosphorylated, active or inactive forms); the interaction is necessary for the complex to interact with alpha-tubulin. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with BBIP10, CBFA2T3, CYLD, DDIT3/CHOP, ZMYND15, F-actin and HDAC11. Interacts with RIPOR2; this interaction occurs during early myogenic differentiation and prevents HDAC6 to deacetylate tubulin. Interacts with DYSF; this interaction occurs during early myogenic differentiation. Interacts with TPPP; inhibiting the tubulin deacetylase activity of HDAC6. Interacts with DYNLL1.
Belongs to the histone deacetylase family. HD type 2 subfamily.
研究領域
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
参考文献
Application: WB Species: mouse Sample: primary neuronal cultures
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