CXCL16 Antibody - #DF13312

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製品: CXCL16 Antibody
カタログ: DF13312
タンパク質の説明: Rabbit polyclonal antibody to CXCL16
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 27kDa, 45 kDa; 28kD(Calculated).
ユニプロット: Q9H2A7
RRID: AB_2846331

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
CXCL16 Antibody detects endogenous levels of total CXCL16.
RRID:
AB_2846331
引用形式: Affinity Biosciences Cat# DF13312, RRID:AB_2846331.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C-X-C motif chemokine 16; Chemokine (C X C motif) ligand 16; Chemokine, CXC motif, ligand 16; CXC chemokine ligand 16; Cxcl16; CXCLG16; CXL16_HUMAN; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small inducible cytokine B16 precursor; Small-inducible cytokine B16; SR-PSOX; SRPSOX; Transmembrane chemokine CXCL16; UNQ2759/PRO6714;

免疫原

免疫原:
Uniprot:

Q9H2A7(CXL16_HUMAN) >>Visit HPA database.

遺伝子(ID):
CXCL16(58191)
発現特異性:
Q9H2A7 CXL16_HUMAN:

Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.

タンパク質配列:
MGRDLRPGSRVLLLLLLLLLVYLTQPGNGNEGSVTGSCYCGKRISSDSPPSVQFMNRLRKHLRAYHRCLYYTRFQLLSWSVCGGNKDPWVQELMSCLDLKECGHAYSGIVAHQKHLLPTSPPISQASEGASSDIHTPAQMLLSTLQSTQRPTLPVGSLSSDKELTRPNETTIHTAGHSLAAGPEAGENQKQPEKNAGPTARTSATVPVLCLLAIIFILTAALSYVLCKRRRGQSPQSSPDLPVHYIPVAPDSNT

PTMs - Q9H2A7 基板として

Site PTM Type Enzyme
S45 Phosphorylation
S46 Phosphorylation
S48 Phosphorylation
S95 Phosphorylation

研究背景

機能:

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

PTMs:

Glycosylated.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein. Secreted.
Note: Also exists as a soluble form.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.

タンパク質ファミリー:

Belongs to the intercrine alpha (chemokine CxC) family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

参考文献

1). Traditional Chinese Medicine Shi-Bi-Man ameliorates psoriasis via inhibiting IL-23/Th17 axis and CXCL16-mediated endothelial activation. Chinese medicine, 2024 (PubMed: 38429819) [IF=4.9]

Application: IF/ICC    Species: Human    Sample: T cells

Fig. 7 SBM inhibits endothelial cells inflammatory response through Cxcl16. A Heatmap for CXCL signaling network. B Dotplot for the expression of genes related to CXCL signaling in endothelial cells and keratinocytes. C Immunofluorescence images staining for CD31 (green), CXCL16 (red), and DAPI (blue) of skin tissue, scale bar = 20 μm
2). A Mouse Model of Damp-Heat Syndrome in Traditional Chinese Medicine and Its Impact on Pancreatic Tumor Growth. Frontiers in Oncology, 2022 (PubMed: 35957897) [IF=4.7]

Application: WB    Species: Mice    Sample: tumor tissues

Figure 6 Chemokine differences between tumor-bearing mice with and without damp-heat syndrome. (A) Heatmap indicating serum chemokine distribution between the two groups (n = 10 in the T group, n = 9 in the TD group). (B) Heatmap indicating tumor tissue chemokine distribution between the two groups (n = 3 in each group). (C) Relative mRNA expression of tissue chemokines tested by qPCR (n = 10 in the T group, n = 9 in the TD group). (D) Relative protein expression of tissue chemokines tested by western blotting (n = 8 in each group). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the T group.
3). Ginsenoside Rg1 Improves Inflammation and Autophagy of the Pancreas and Spleen in Streptozotocin-Induced Type 1 Diabetic Mice. International Journal of Endocrinology, 2023 (PubMed: 36960388) [IF=2.8]

Application: IF/ICC    Species: Mouse    Sample:

Figure 3 Expressions of CXCL16, CD45, and insulin receptor (INSR) proteins in the pancreas by immunofluorescence (×400, scale: 50 μm, mean ± SD, n = 6) (a–c) the expressions of CXCL16 and CD45 proteins' colocalization in the pancreas. (d–f) The expressions of INSR and CXCL16 proteins colocalization in the pancreas. The fluorescence intensity was detected to observe the protein expression. The increase in CD45 indicated an increase in inflammatory cell infiltration. Those results suggested that Rg1 treatment improves inflammation of the pancreas in type 1 diabetes mice relating to reducing CXCL16. ∗∗p < 0.01, vs. the control group; ##p < 0.01 vs. the DM group.

Application: WB    Species: Mouse    Sample:

Figure 8 The expression of CXCL16, NF-κB, TF, LC3, and P62 proteins in the pancreas and spleens. (Mean ± SD, n = 3) (a–f) the expression of CXCL16, NF-κB, TF, LC3, and P62 proteins in the pancreas. (g–l) The expression of CXCL16, NF-κB, TF, LC3, and P62 proteins in the spleens. The improvement of Rg1 treatment on type 1 diabetes mice was related to the increase of CXCL16, NF-κB, and TF and the raised autophagy (IL3 II/LC 3 I). ∗∗p < 0.01, vs. the control group; ns p > 0.05, ##p < 0.01 vs. the DM group.
4). YAP1/MMP7/CXCL16 axis affects efficacy of neoadjuvant chemotherapy via tumor environment immunosuppression in triple-negative breast cancer. Gland Surgery, 2021 (PubMed: 34733729) [IF=1.8]

Application: WB    Species: Human    Sample: HCC70 cell

Figure 4 Western blotting assay was employed to measure cytoplasmic YAP1 as well as soluble MMP7 and CXCL16 in extracellular matrix in MDA-MB-231 and HCC70 cell lines, respectively. Modulation sequence of protein expression was estimated via YAP1 inhibitor (verteporfin) and MMPs inhibitor (GM6001). Results were statistically analyzed

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