Phospho-DARPP32 (Thr75) Antibody - #AF3487
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製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3487, RRID:AB_2834925.
折りたたみ／展開
DARPP 32; DARPP-32; Dopamine and cAMP regulated neuronal phosphoprotein 32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32; Dopamine and cAMP regulated phosphoprotein DARPP32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; PPR1B_HUMAN; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory inhibitor subunit 1B; Protein phosphatase 1 regulatory subunit 1B;
免疫原
- Q9UD71 PPR1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPAMLFRLSEHSSPEEEASPHQRASGEGHHLKSKRPNPCAYTPPSLKAVQRIAESHLQSISNLNENQASEEEDELGELRELGYPREEDEEEEEDDEEEEEEEDSQAEVLKVIRQSAGQKTTCGQGLEGPWERPPPLDESERDGGSEDQVEDPALSEPGEEPQRPSPSEPGT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UD71 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T34 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S45 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| Y74 | Phosphorylation | Uniprot | |
| T75 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S78 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot |
研究背景
Inhibitor of protein-phosphatase 1.
Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.
Cytoplasm.
Belongs to the protein phosphatase inhibitor 1 family.
研究領域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Cocaine addiction.
· Human Diseases > Substance dependence > Amphetamine addiction.
· Human Diseases > Substance dependence > Alcoholism.
· Organismal Systems > Nervous system > Dopaminergic synapse.
参考文献
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