STIL Antibody - #DF13333
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13333, RRID:AB_2846352.
折りたたみ／展開
DKFZp686O09161; MCPH7; OTTHUMP00000009566; SCL interrupting locus protein; SCL-interrupting locus protein; SCL/TAL1 interrupting locus; SIL; STIL; STIL_HUMAN; TAL 1 interrupting locus protein; TAL-1-interrupting locus protein;
免疫原
Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
- Q15468 STIL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPIYPFARPQMNTRFPSSRMVPFHFPPSKCALWNPTPTGDFIYLHLSYYRNPKLVVTEKTIRLAYRHAKQNKKNSSCFLLGSLTADEDEEGVTLTVDRFDPGREVPECLEITPTASLPGDFLIPCKVHTQELCSREMIVHSVDDFSSALKALQCHICSKDSLDCGKLLSLRVHITSRESLDSVEFDLHWAAVTLANNFKCTPVKPIPIIPTALARNLSSNLNISQVQGTYKYGYLTMDETRKLLLLLESDPKVYSLPLVGIWLSGITHIYSPQVWACCLRYIFNSSVQERVFSESGNFIIVLYSMTHKEPEFYECFPCDGKIPDFRFQLLTSKETLHLFKNVEPPDKNPIRCELSAESQNAETEFFSKASKNFSIKRSSQKLSSGKMPIHDHDSGVEDEDFSPRPIPSPHPVSQKISKIQPSVPELSLVLDGNFIESNPLPTPLEMVNNENPPLINHLEHLKPLQPQLYDEKHSPEVEAGEPSLRGIPNQLNQDKPALLRHCKVRQPPAYKKGNPHTRNSIKPSSHNGPSHDIFEKLQTVSAGNVQNEEYPIRPSTLNSRQSSLAPQSQPHDFVFSPHNSGRPMELQIPTPPLPSYCSTNVCRCCQHHSHIQYSPLNSWQGANTVGSIQDVQSEALQKHSLFHPSGCPALYCNAFCSSSSPIALRPQGDMGSCSPHSNIEPSPVARPPSHMDLCNPQPCTVCMHTPKTESDNGMMGLSPDAYRFLTEQDRQLRLLQAQIQRLLEAQSLMPCSPKTTAVEDTVQAGRQMELVSVEAQSSPGLHMRKGVSIAVSTGASLFWNAAGEDQEPDSQMKQDDTKISSEDMNFSVDINNEVTSLPGSASSLKAVDIPSFEESNIAVEEEFNQPLSVSNSSLVVRKEPDVPVFFPSGQLAESVSMCLQTGPTGGASNNSETSEEPKIEHVMQPLLHQPSDNQKIYQDLLGQVNHLLNSSSKETEQPSTKAVIISHECTRTQNVYHTKKKTHHSRLVDKDCVLNATLKQLRSLGVKIDSPTKVKKNAHNVDHASVLACISPEAVISGLNCMSFANVGMSGLSPNGVDLSMEANAIALKYLNENQLSQLSVTRSNQNNCDPFSLLHINTDRSTVGLSLISPNNMSFATKKYMKRYGLLQSSDNSEDEEEPPDNADSKSEYLLNQNLRSIPEQLGGQKEPSKNDHEIINCSNCESVGTNADTPVLRNITNEVLQTKAKQQLTEKPAFLVKNLKPSPAVNLRTGKAEFTQHPEKENEGDITIFPESLQPSETLKQMNSMNSVGTFLDVKRLRQLPKLF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15468 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| Y470 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| K512 | Acetylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| T591 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S753 | Phosphorylation | Uniprot | |
| K755 | Ubiquitination | Uniprot | |
| S779 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| S869 | Phosphorylation | Uniprot | |
| S871 | Phosphorylation | Uniprot | |
| S873 | Phosphorylation | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| K936 | Ubiquitination | Uniprot | |
| Y938 | Phosphorylation | Uniprot | |
| K954 | Ubiquitination | Uniprot | |
| K962 | Ubiquitination | Uniprot | |
| K991 | Ubiquitination | Uniprot | |
| K1000 | Ubiquitination | Uniprot | |
| S1004 | Phosphorylation | Uniprot | |
| K1008 | Ubiquitination | Uniprot | |
| S1011 | Phosphorylation | Uniprot | |
| K1014 | Ubiquitination | Uniprot | |
| S1061 | Phosphorylation | Uniprot | |
| S1108 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1111 | Phosphorylation | Uniprot | |
| S1116 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T1119 | Phosphorylation | Uniprot | |
| K1120 | Ubiquitination | Uniprot | |
| S1132 | Phosphorylation | Uniprot | |
| S1135 | Phosphorylation | Uniprot | |
| K1168 | Ubiquitination | Uniprot | |
| K1206 | Ubiquitination | Uniprot | |
| K1214 | Ubiquitination | Uniprot | |
| K1223 | Ubiquitination | Uniprot | |
| S1225 | Phosphorylation | Uniprot | |
| K1234 | Ubiquitination | Uniprot | |
| K1243 | Ubiquitination | Uniprot | |
| T1250 | Phosphorylation | Uniprot |
研究背景
Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. Plays an important role in the regulation of centriole duplication. Required for the onset of procentriole formation and proper mitotic progression. During procentriole formation, is essential for the correct loading of SASS6 and CENPJ to the base of the procentriole to initiate procentriole assembly.
Ubiquitinated.
Phosphorylated following the activation of the mitotic checkpoint.
Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
Homodimer. Interacts with PIN1 via its WW domain. This interaction is dependent on STIL mitotic phosphorylation (By similarity). Interacts with CENPJ. Interacts with RBM14 and this interaction interferes with the interaction of STIL with CENPJ. Forms a complex with CENPJ and SASS6.
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