Glutaminase Antibody - #DF13334
(2)
(5)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13334, RRID:AB_2846353.
折りたたみ／展開
AAD20; DKFZp686O15119; FLJ10358; GAC; GAM; GLS; GLS1; GLSK_HUMAN; Glutaminase C; Glutaminase kidney isoform; Glutaminase phosphate activated; K-glutaminase; KGA; KIAA0838; L-glutamine amidohydrolase; mitochondrial;
免疫原
Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
- O94925 GLSK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMRLRGSGMLRDLLLRSPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLHPWWGGGGWPAEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAPAAPGPKDGPGETDAFGNSEGKELVASGENKIKQGLLPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSTGDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLIKQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O94925 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S17 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K158 | Acetylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K164 | Acetylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K176 | Acetylation | Uniprot | |
| Y249 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| Y304 | Phosphorylation | Uniprot | |
| K311 | Acetylation | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| K320 | Acetylation | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| Y370 | Phosphorylation | Uniprot | |
| R387 | Methylation | Uniprot | |
| Y393 | Phosphorylation | Uniprot | |
| Y394 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| S523 | Phosphorylation | Uniprot |
研究背景
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain.
Lacks catalytic activity.
Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits.
Mitochondrion. Cytoplasm>Cytosol.
Note: The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits.
Mitochondrion.
Mitochondrion matrix.
Note: Produced by the proteolytic processing of the 74-kDa cytosolic precursor.
Mitochondrion matrix.
Note: Produced by the proteolytic processing of the 74-kDa cytosolic precursor.
Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
Homotetramer, dimer of dimers. The tetramers can assemble into rod-like oligomers (in vitro), but the physiological significance of this is not clear (By similarity). Interacts with RAF1 and MAP2K2. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.
The C-terminal ANK repeats prevent the assembly of the supra-tetrameric filaments.
A highly mobile activation loop at the dimer-dimer interface is important for enzyme activity.
Belongs to the glutaminase family.
研究領域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Metabolism > Amino acid metabolism > Arginine biosynthesis.
· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.
· Metabolism > Metabolism of other amino acids > D-Glutamine and D-glutamate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > GABAergic synapse.
· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.
参考文献
Application: WB Species: Human Sample: EC cell
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