SLC31A1 / CTR1 Antibody - #DF13356

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製品: SLC31A1 / CTR1 Antibody
カタログ: DF13356
タンパク質の説明: Rabbit polyclonal antibody to SLC31A1 / CTR1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Horse, Rabbit, Dog
分子量: 21kDa; 21kD(Calculated).
ユニプロット: O15431
RRID: AB_2846375

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Horse(100%), Rabbit(100%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
SLC31A1 / CTR1 Antibody detects endogenous levels of total SLC31A1 / CTR1.
RRID:
AB_2846375
引用形式: Affinity Biosciences Cat# DF13356, RRID:AB_2846375.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Copper transport 1 homolog; Copper transporter 1; COPT1; COPT1_HUMAN; CTR1; hCTR1; High affinity copper uptake protein 1; SLC31A1; solute carrier family 31 (copper tansporters) member 1; Solute carrier family 31 member 1;

免疫原

免疫原:
Uniprot:

O15431(COPT1_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC31A1(1317)
タンパク質配列:
MDHSHHMGMSYMDSNSTMQPSHHHPTTSASHSHGGGDSSMMMMPMTFYFGFKNVELLFSGLVINTAGEMAGAFVAVFLLAMFYEGLKIARESLLRKSQVSIRYNSMPVPGPNGTILMETHKTVGQQMLSFPHLLQTVLHIIQVVISYFLMLIFMTYNGYLCIAVAAGAGTGYFLFSWKKAVVVDITEHCH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Dog
88
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15431 基板として

Site PTM Type Enzyme
S4 Phosphorylation
S10 Phosphorylation
Y11 Phosphorylation
N15 N-Glycosylation
T27 O-Glycosylation
K96 Ubiquitination
S105 Phosphorylation
T114 Phosphorylation

研究背景

機能:

High-affinity, saturable copper transporter involved in dietary copper uptake.

PTMs:

O-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.
Note: Localizes to the apical membrane in intestinal epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homotrimer.

タンパク質ファミリー:

Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.

研究領域

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Organismal Systems > Digestive system > Mineral absorption.

参考文献

1). Comprehensive Gene Analysis Reveals Cuproptosis-Related Gene Signature Associated with M2 Macrophage in Staphylococcus aureus-Infected Osteomyelitis. Journal of inflammation research, 2024 (PubMed: 38770176) [IF=4.5]
2). Bioinformatics analysis and experimental validation of m6A and cuproptosis-related lncRNA NFE4 in clear cell renal cell carcinoma. Discover oncology, 2024 (PubMed: 38797784) [IF=2.2]

Application: WB    Species: Human    Sample: Caki-1 and OS-RC-2 cells

Figure. 9 Functional experiments against NFE4 in Caki-1 and OS-RC-2 cells. A NFE4 was highly expressed in tumor tissues. B The expression of NFE4 in renal cancer cells was measured using real-time quantitative reverse transcription-PCR. C Survival analysis of the high NFE4 group and low NFE4 group. D, E The mRNA expression of NFE4 in Caki-1 and OS-RC-2 cells was measured using real-time quantitative reverse transcription-PCR. F, G Cell Counting Kit-8 assays were used to detect the effect of NFE4 knockdown on cell proliferation in Caki-1 and OS-RC-2 cells. H qRT-PCR shows the expression and regulation of NFE4 in Caki-1 and OS-RC-2 cells treated with drugs that induce cuproptosis for 24 h (n = 3). CuCl2 (2mM), Elesclomol (20 nM), both CuCl2 (2mM) and Elesclomol (20 nM). I Western blotting was used to determine the protein levels of ATP7B and SLC31A1 in Caki-1 and OS-RC-2 cells. One-way ANOVA, NC was used as a control group. Data are shown as the mean ± standard deviation from three independent experiments and were compared to the respective si-NC group. ****P < 0.0001; ***P < 0.001; **P < 0.01 and *P < 0.05.

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