DFFB Antibody - #DF13360

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製品: DFFB Antibody
カタログ: DF13360
タンパク質の説明: Rabbit polyclonal antibody to DFFB
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog
分子量: 39kDa; 39kD(Calculated).
ユニプロット: O76075
RRID: AB_2846379

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(88%), Zebrafish(83%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
DFFB Antibody detects endogenous levels of total DFFB.
RRID:
AB_2846379
引用形式: Affinity Biosciences Cat# DF13360, RRID:AB_2846379.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CAD; Caspase activated deoxyribonuclease; Caspase activated DNase; Caspase activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; Caspase-activated nuclease; CPAN; Deoxyribonuclease III, caspase activated; DFF 40; DFF-40; DFF2; DFF40; Dffb; DFFB_HUMAN; Didff; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase); DNA fragmentation factor, 40 kD beta subunit; DNA fragmentation factor, 40 kD, beta polypeptide; DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase); OTTHUMP00000003633;

免疫原

免疫原:
Uniprot:

O76075(DFFB_HUMAN) >>Visit HPA database.

遺伝子(ID):
DFFB(1677)
タンパク質配列:
MLQKPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQGYVSDIRRFLSAFHEPQVGLIQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTVGAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNLDHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTHKLNCDPSRIYKPQTRLKRKQPVRKRQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Zebrafish
83
Xenopus
75
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O76075 基板として

Site PTM Type Enzyme
K152 Ubiquitination
Y158 Phosphorylation
K264 Ubiquitination
K265 Ubiquitination
K277 Ubiquitination
S319 Phosphorylation
Y322 Phosphorylation
K323 Ubiquitination
T326 Phosphorylation

研究背景

機能:

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Heterodimer of DFFA and DFFB. Interacts with H1-1.

研究領域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

参考文献

1). Downregulation of the Long Noncoding RNA IALNCR Targeting MAPK8/JNK1 Promotes Apoptosis and Antagonizes Bovine Viral Diarrhea Virus Replication in Host Cells. Journal of Virology, 2022 (PubMed: 35993735) [IF=5.4]

Application: WB    Species: Bovine    Sample: MDBK cells and BT cell

FIG 3 mRNA levels of apoptosis-related genes caspase-3 and Bcl-2 (A), and Bax and Bak1 (B) detected by qRT-PCR at different time points after BVDV infection (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (C) Protein levels of caspase-3 and Bcl-2 were detected by Western blotting at different time points after BVDV infection. Lowercase letters (a versus b; b versus c) indicate a significant difference of P < 0.05; a versus c indicates a significant difference of P < 0.01. (D) Protein levels of apoptosis-related pathway proteins were detected by Western blotting (***, P < 0.001). (E) Identification of procaspase-3/8/9 cleavage, ICAD/DFF45 degradation, nuclear translocation of active caspase-3 and CAD/DFF40, and nuclear PARP cleavage by Western blotting in BVDV-infected MDBK cells and BT cells at different time points after infection.

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