XLF Antibody - #DF13366
| 製品: | XLF Antibody |
| カタログ: | DF13366 |
| タンパク質の説明: | Rabbit polyclonal antibody to XLF |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Xenopus |
| 分子量: | 33kDa; 33kD(Calculated). |
| ユニプロット: | Q9H9Q4 |
| RRID: | AB_2846385 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13366, RRID:AB_2846385.
折りたたみ／展開
Cernunnos; FLJ12610; NHEJ 1; Nhej1; NHEJ1, S. cerevisiae, homolog of; NHEJ1_HUMAN; Non homologous end joining factor 1; Non-homologous end-joining factor 1; Nonhomologous end joining factor 1; OTTHUMP00000164168; OTTHUMP00000206275; OTTHUMP00000206279; Protein cernunnos; XLF; XRCC4 like factor; XRCC4-like factor;
免疫原
- Q9H9Q4 NHEJ1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAAFLCHLDNLLRPLLKDAAHPSEATFSCDCVADALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLMGMSLALQCQVRELATLLHMKDLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPFVMNLQDLYMAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTGTSGPLQRPQLSKVKRKKPRGLFS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H9Q4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S55 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S132 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| K180 | Ubiquitination | Uniprot | |
| T181 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S203 | Phosphorylation | Uniprot | |
| T223 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S245 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S251 | Phosphorylation | Q13315 (ATM) , P78527 (PRKDC) | Uniprot |
| S262 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T266 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S268 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| K288 | Methylation | Uniprot |
研究背景
DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Binds DNA in a length-dependent manner.
Nucleus.
Ubiquitously expressed.
Exists mainly as a homodimer. Associates with the non-homologous end joining (NHEJ) complex which is at least composed of the core proteins XRCC5/Ku80, XRCC6/Ku70, PRKDC/DNA-PKcs, LIG4 and XRCC4.
The coiled-coil region mediates homodimerization.
Belongs to the XLF family.
研究領域
· Genetic Information Processing > Replication and repair > Non-homologous end-joining.
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