Phospho-HDAC5 (Ser498) Antibody - #AF3491

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製品: Phospho-HDAC5 (Ser498) Antibody
カタログ: AF3491
タンパク質の説明: Rabbit polyclonal antibody to Phospho-HDAC5 (Ser498)
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 124kDa; 122kD(Calculated).
ユニプロット: Q9UQL6
RRID: AB_2834929

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-HDAC5 (Ser498) Antibody detects endogenous levels of HDAC5 only when phosphorylated at Serine 498.
RRID:
AB_2834929
引用形式: Affinity Biosciences Cat# AF3491, RRID:AB_2834929.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Antigen NY CO 9; Antigen NY-CO-9; HD5; HDAC 5; HDAC5; HDAC5_HUMAN; Histone deacetylase 5; NY CO 9;

免疫原

免疫原:

A synthesized peptide derived from human HDAC5 around the phosphorylation site of Ser498.

Uniprot:

Q9UQL6(HDAC5_HUMAN) >>Visit HPA database.

遺伝子(ID):
HDAC5(10014)
発現特異性:
Q9UQL6 HDAC5_HUMAN:

Ubiquitous.

タンパク質の説明:
HDAC9 a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4.
タンパク質配列:
MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
91
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.

PTMs:

Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription (By similarity).

Ubiquitinated. Polyubiquitination however does not lead to its degradation.

細胞の位置付け:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

タンパク質ファミリー:

The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.

Belongs to the histone deacetylase family. HD type 2 subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

参考文献

1). Wilson disease: intersecting DNA methylation and histone acetylation regulation of gene expression in a mouse model of hepatic copper accumulation. Cellular and Molecular Gastroenterology and Hepatology, 2021 (PubMed: 34098115) [IF=7.1]

Application: WB    Species: Mice    Sample: tx-j

Figure 2. Expression of HDAC4 and HDAC5 in mouse and HepG2 models of WD. Immunoblot densitometry analyses are normalized to β-ACTIN; data are represented as means ± SEM and statistical significance was determined by Student's t-test (*p< 0.05, **p< 0.01, and ****p< 0.0001). A: Total protein liver lysate densitometries for HDAC4 and HDAC5 protein expression in tx-j mice compared to C3H control at post-partum day 6 (PPD6; C3H n=4M/4F, tx-j n=5M/4F), 9 weeks (C3H n=4M/3F, tx-j n=4M/4F), 12 weeks (C3H n=4M/4F, tx-j n=4M/3F), and 24 weeks (C3H n=10M/12F, tx-j n=11M/11F). B: Total protein liver lysate densitometry analyses for HDAC4 and HDAC5 protein expression in 16-week old Atp7b -/- mice (n=7M/4F) and wild-type (WT, n=6M/5F). C: Immunohistochemical analysis of 24-week old C3H and tx-j mouse livers for HDAC5 (red) and DAPI (blue). Images display cytosolic and nuclear HDAC5 localization. White arrows indicate nuclei; scale bar = 50 µm. Bar graphs represent HDAC5 optical density in cytosol, nuclei, and the nucleus/cytosol ratio; n=3 mice/group, 60 cells/mouse. D: HDAC5 immunoblot of HepG2 cell lysates treated with CuSO 4 (0 to 100 µM; n=3 per treatment) for 24 hours. In this figure and all subsequent figures: C3H=C3HeB/FeJ control mice with normal copper metabolism, tx-j=C3He- Atp7b tx-j /J Jackson Laboratory toxic milk mouse model of Wilson disease (WD).
2). Antidepressant effect and mechanism of TMP269 on stress-induced depressive-like behavior in mice. Biochemical pharmacology, 2024 (PubMed: 38801927) [IF=5.3]
3). WNT10A induces apoptosis of senescent synovial resident stem cells through Wnt/calcium pathway-mediated HDAC5 phosphorylation in OA joints. BONE, 2021 (PubMed: 34000432) [IF=3.5]

Application: WB    Species: human    Sample: Sn-SMSCs

Fig. 4. |Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phosphorylation at s498 (right); *P<0.05

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