EST1A Antibody - #DF13372
| 製品: | EST1A Antibody |
| カタログ: | DF13372 |
| タンパク質の説明: | Rabbit polyclonal antibody to EST1A |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 160kDa; 160kD(Calculated). |
| ユニプロット: | Q86US8 |
| RRID: | AB_2846391 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13372, RRID:AB_2846391.
折りたたみ／展開
C17orf31; EST1-like protein A; Ever shorter telomeres 1A; hSmg5/7a; KIAA0732; Smg-6 homolog; SMG6; Telomerase subunit EST1A; Telomerase-binding protein EST1A;
免疫原
- Q86US8 EST1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEGLERVRISASELRGILATLAPQAGSRENMKELKEARPRKDNRRPDLEIYKPGLSRLRNKPKIKEPPGSEEFKDEIVNDRDCSAVENGTQPVKDVCKELNNQEQNGPIDPENNRGQESFPRTAGQEDRSLKIIKRTKKPDLQIYQPGRRLQTVSKESASRVEEEEVLNQVEQLRVEEDECRGNVAKEEVANKPDRAEIEKSPGGGRVGAAKGEKGKRMGKGEGVRETHDDPARGRPGSAKRYSRSDKRRNRYRTRSTSSAGSNNSAEGAGLTDNGCRRRRQDRTKERPRLKKQVSVSSTDSLDEDRIDEPDGLGPRRSSERKRHLERNWSGRGEGEQKNSAKEYRGTLRVTFDAEAMNKESPMVRSARDDMDRGKPDKGLSSGGKGSEKQESKNPKQELRGRGRGILILPAHTTLSVNSAGSPESAPLGPRLLFGSGSKGSRSWGRGGTTRRLWDPNNPDQKPALKTQTPQLHFLDTDDEVSPTSWGDSRQAQASYYKFQNSDNPYYYPRTPGPASQYPYTGYNPLQYPVGPTNGVYPGPYYPGYPTPSGQYVCSPLPTSTMSPEEVEQHMRNLQQQELHRLLRVADNQELQLSNLLSRDRISPEGLEKMAQLRAELLQLYERCILLDIEFSDNQNVDQILWKNAFYQVIEKFRQLVKDPNVENPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLEDYMDGLAIRSKPLRKTVKYALISAQRCMICQGDIARYREQASDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKAEQMEKKQHEEFDLSPDQWRKGKKSTFRHVGDDTTRLEIWIHPSHPRSSQGTESGKDSEQENGLGSLSPSDLNKRFILSFLHAHGKLFTRIGMETFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAAALGLAMFSLLVRRCTCLLKESAKAQLSSPEDQDDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPSHVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGTRLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDHSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLEQRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDFMPASKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86US8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| R404 | Methylation | Uniprot | |
| R406 | Methylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S440 | Phosphorylation | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T471 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| T486 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| Y508 | Phosphorylation | Uniprot | |
| Y509 | Phosphorylation | Uniprot | |
| R512 | Methylation | Uniprot | |
| Y769 | Phosphorylation | Uniprot | |
| Y778 | Phosphorylation | Uniprot | |
| Y788 | Phosphorylation | Uniprot | |
| Y789 | Phosphorylation | Uniprot | |
| S796 | Phosphorylation | Uniprot | |
| K803 | Acetylation | Uniprot | |
| K814 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| S831 | Phosphorylation | Uniprot | |
| S864 | Phosphorylation | Uniprot | |
| S865 | Phosphorylation | Uniprot | |
| T868 | Phosphorylation | Uniprot | |
| S870 | Phosphorylation | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| S884 | Phosphorylation | Uniprot | |
| K918 | Acetylation | Uniprot | |
| K995 | Ubiquitination | Uniprot | |
| S1000 | Phosphorylation | Uniprot | |
| K1011 | Ubiquitination | Uniprot | |
| K1149 | Ubiquitination | Uniprot | |
| K1225 | Ubiquitination | Uniprot | |
| K1309 | Ubiquitination | Uniprot | |
| K1376 | Ubiquitination | Uniprot | |
| T1390 | Phosphorylation | Uniprot |
研究背景
Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER).
Plays a role in nonsense-mediated mRNA decay. Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA.
Nucleus>Nucleolus. Chromosome>Telomere. Cytoplasm>Cytosol.
Note: Particularly enriched in the nucleolus.
Ubiquitous.
May form homooligomers. Associated component of the telomerase holoenzyme complex. Interacts with TERT, independently of the telomerase RNA. Interacts with SMG1, SMG5, SMG7, UPF1, UPF2, UPF3B and the PP2A catalytic subunits. Also interacts with the exon junction complex (EJC) composed at least of CASC3, EIF4A3, MAGOH and RBM8A; required for the process of nonsense-mediated mRNA decay.
The PINc domain confers endonuclease activity and is expected to bind the catalytic metal ion.
研究領域
· Genetic Information Processing > Translation > mRNA surveillance pathway.
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