hSET1 Antibody - #DF13382

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製品: hSET1 Antibody
カタログ: DF13382
タンパク質の説明: Rabbit polyclonal antibody to hSET1
アプリケーション: WB
反応性: Human
予測: Pig, Bovine, Horse, Dog
分子量: 186kDa; 186kD(Calculated).
ユニプロット: O15047
RRID: AB_2846401

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
hSET1 Antibody detects endogenous levels of total hSET1.
RRID:
AB_2846401
引用形式: Affinity Biosciences Cat# DF13382, RRID:AB_2846401.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Histone-lysine N-methyltransferase SETD1A; hSET1A; KMT2F; Lysine N methyltransferase 2F; Lysine N-methyltransferase 2F; SET domain containing 1A; SET domain containing protein 1A; SET domain-containing protein 1A; SET1; Set1 Ash2 histone methyltransferase complex subunit SET1; Set1/Ash2 histone methyltransferase complex subunit SET1; SET1A; SET1A_HUMAN; SETD1A;

免疫原

免疫原:
Uniprot:

O15047(SET1A_HUMAN) >>Visit HPA database.

遺伝子(ID):
SETD1A(9739)
タンパク質配列:
MDQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVNDSKYIPVEDLQDPRCHVRSKNRDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARVLFTSTRGAKETVKNLHLTSVMGNIIHAQLDIKGQQRMKYYELIVNGSYTPQTVPTGGKALSEKFQGSGAATETAESRRRSSSDTAAYPAGTTAVGTPGNGTPCSQDTSFSSSRQDTPSSFGQFTPQSSQGTPYTSRGSTPYSQDSAYSSSTTSTSFKPRRSENSYQDAFSRRHFSASSASTTASTAIAATTAATASSSASSSSLSSSSSSSSSSSSSQFRSSDANYPAYYESWNRYQRHTSYPPRRATREEPPGAPFAENTAERFPPSYTSYLPPEPSRPTDQDYRPPASEAPPPEPPEPGGGGGGGGPSPEREEVRTSPRPASPARSGSPAPETTNESVPFAQHSSLDSRIEMLLKEQRSKFSFLASDTEEEEENSSMVLGARDTGSEVPSGSGHGPCTPPPAPANFEDVAPTGSGEPGATRESPKANGQNQASPCSSGDDMEISDDDRGGSPPPAPTPPQQPPPPPPPPPPPPPYLASLPLGYPPHQPAYLLPPRPDGPPPPEYPPPPPPPPHIYDFVNSLELMDRLGAQWGGMPMSFQMQTQMLTRLHQLRQGKGLIAASAGPPGGAFGEAFLPFPPPQEAAYGLPYALYAQGQEGRGAYSREAYHLPMPMAAEPLPSSSVSGEEARLPPREEAELAEGKTLPTAGTVGRVLAMLVQEMKSIMQRDLNRKMVENVAFGAFDQWWESKEEKAKPFQNAAKQQAKEEDKEKTKLKEPGLLSLVDWAKSGGTTGIEAFAFGSGLRGALRLPSFKVKRKEPSEISEASEEKRPRPSTPAEEDEDDPEQEKEAGEPGRPGTKPPKRDEERGKTQGKHRKSFALDSEGEEASQESSSEKDEEDDEEDEEDEDREEAVDTTKKETEVSDGEDEESDSSSKCSLYADSDGENDSTSDSESSSSSSSSSSSSSSSSSSSSSSSSESSSEDEEEEERPAALPSASPPPREVPVPTPAPVEVPVPERVAGSPVTPLPEQEASPARPAGPTEESPPSAPLRPPEPPAGPPAPAPRPDERPSSPIPLLPPPKKRRKTVSFSAIEVVPAPEPPPATPPQAKFPGPASRKAPRGVERTIRNLPLDHASLVKSWPEEVSRGGRSRAGGRGRLTEEEEAEPGTEVDLAVLADLALTPARRGLPALPAVEDSEATETSDEAERPRPLLSHILLEHNYALAVKPTPPAPALRPPEPVPAPAALFSSPADEVLEAPEVVVAEAEEPKPQQLQQQREEGEEEGEEEGEEEEEESSDSSSSSDGEGALRRRSLRSHARRRRPPPPPPPPPPRAYEPRSEFEQMTILYDIWNSGLDSEDMSYLRLTYERLLQQTSGADWLNDTHWVHHTITNLTTPKRKRRPQDGPREHQTGSARSEGYYPISKKEKDKYLDVCPVSARQLEGVDTQGTNRVLSERRSEQRRLLSAIGTSAIMDSDLLKLNQLKFRKKKLRFGRSRIHEWGLFAMEPIAADEMVIEYVGQNIRQMVADMREKRYVQEGIGSSYLFRVDHDTIIDATKCGNLARFINHCCTPNCYAKVITIESQKKIVIYSKQPIGVDEEITYDYKFPLEDNKIPCLCGTESCRGSLN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
67
Sheep
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15047 基板として

Site PTM Type Enzyme
K21 Ubiquitination
T97 Phosphorylation
K111 Ubiquitination
Y179 Phosphorylation
Y180 Phosphorylation
Y188 Phosphorylation
S207 Phosphorylation
S220 Phosphorylation
S221 Phosphorylation
S222 Phosphorylation
T224 Phosphorylation
S268 Phosphorylation
T271 Phosphorylation
Y273 Phosphorylation
T274 Phosphorylation
R276 Methylation
S282 Phosphorylation
S293 Phosphorylation
S315 Phosphorylation
T330 Phosphorylation
S343 Phosphorylation
S347 Phosphorylation
S348 Phosphorylation
S349 Phosphorylation
S350 Phosphorylation
S351 Phosphorylation
S352 Phosphorylation
Y366 Phosphorylation
Y370 Phosphorylation
T388 Phosphorylation
Y409 Phosphorylation
S450 Phosphorylation
S459 Phosphorylation
S464 Phosphorylation
S468 Phosphorylation
S470 Phosphorylation
T476 Phosphorylation
S501 Phosphorylation
S504 Phosphorylation
S508 Phosphorylation
T510 Phosphorylation
S534 Phosphorylation
T540 Phosphorylation
T562 Phosphorylation
S565 Phosphorylation
S586 Phosphorylation
K803 Acetylation
S882 Phosphorylation
S892 Phosphorylation
K898 Acetylation
S904 Phosphorylation
S907 Phosphorylation
K910 Acetylation
S915 Phosphorylation
T916 Phosphorylation
S958 Phosphorylation
S963 Phosphorylation
S969 Phosphorylation
S972 Phosphorylation
S973 Phosphorylation
S1004 Phosphorylation
S1037 Phosphorylation
S1038 Phosphorylation
S1039 Phosphorylation
S1040 Phosphorylation
S1041 Phosphorylation
S1046 Phosphorylation
S1047 Phosphorylation
S1048 Phosphorylation
S1049 Phosphorylation
T1088 Phosphorylation
S1103 Phosphorylation
T1106 Phosphorylation
S1114 Phosphorylation
T1167 Phosphorylation
S1169 Phosphorylation
S1171 Phosphorylation
T1185 Phosphorylation
T1206 Phosphorylation
S1216 Phosphorylation
R1227 Methylation
S1545 Phosphorylation
T1549 Phosphorylation
S1550 Phosphorylation
K1637 Ubiquitination

研究背景

機能:

Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.

細胞の位置付け:

Nucleus speckle. Chromosome.
Note: Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with HCFC1. Interacts with ASH2/ASH2L, CXXC1/CFP1 and RBBP5. Interacts (via the RRM domain) with WDR82. Interacts (via the RRM domain) with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A) only in the presence of WDR82. Binds specifically to CTD heptad repeats phosphorylated on 'Ser-5' of each heptad. Interacts with ZNF335. Interacts with SUPT6H. Interacts with NAP1L1 (By similarity). Interacts (via WIN motif) with WDR5.

タンパク質ファミリー:

Belongs to the class V-like SAM-binding methyltransferase superfamily.

研究領域

· Metabolism > Amino acid metabolism > Lysine degradation.

参考文献

1). METTL3 preferentially enhances non-m6A translation of epigenetic factors and promotes tumourigenesis. Nature Cell Biology, 2022 (PubMed: 35927451) [IF=21.3]

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