製品: Transferrin Antibody
カタログ: DF13383
タンパク質の説明: Rabbit polyclonal antibody to Transferrin
アプリケーション: WB IF/ICC
反応性: Human
予測: Rabbit
分子量: 77kDa; 77kD(Calculated).
ユニプロット: P02787
RRID: AB_2846402

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Rabbit(86%)
クローナリティ:
Polyclonal
特異性:
Transferrin Antibody detects endogenous levels of total Transferrin.
RRID:
AB_2846402
引用形式: Affinity Biosciences Cat# DF13383, RRID:AB_2846402.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Apotransferrin; Beta 1 metal binding globulin; Beta-1 metal-binding globulin; DKFZp781D0156; PRO1400; PRO1557; PRO2086; Serotransferrin; Serotransferrin precursor; Siderophilin; TF; TFQTL1; Transferin; Transferrin; TRFE_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P02787 TRFE_HUMAN:

Expressed by the liver and secreted in plasma.

タンパク質配列:
MRLAVGALLVCAVLGLCLAVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
86
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02787 基板として

Site PTM Type Enzyme
T36 Phosphorylation
K37 Acetylation
C58 S-Nitrosylation
S63 Phosphorylation
Y64 Phosphorylation
S136 Phosphorylation
C137 S-Nitrosylation
T139 Phosphorylation
S144 Phosphorylation
Y155 Phosphorylation
Y207 Phosphorylation
C213 S-Nitrosylation
C246 S-Nitrosylation
Y257 Phosphorylation
C260 S-Nitrosylation
S298 Phosphorylation
S305 Phosphorylation
S306 Phosphorylation
T340 Phosphorylation
T349 Phosphorylation
T355 Phosphorylation
K359 Acetylation
C364 S-Nitrosylation
C374 S-Nitrosylation
S389 Phosphorylation
C396 S-Nitrosylation
S468 Phosphorylation
T476 Phosphorylation
N491 N-Glycosylation
C503 S-Nitrosylation
K508 Acetylation
S520 Phosphorylation
C525 S-Nitrosylation
Y533 Phosphorylation
Y534 Phosphorylation
Y536 Phosphorylation
T537 Phosphorylation
K546 Acetylation
C582 S-Nitrosylation
Y593 Phosphorylation
C596 S-Nitrosylation
T645 Phosphorylation
T654 Phosphorylation
Y666 Phosphorylation
Y669 Phosphorylation
Y674 Phosphorylation
C684 S-Nitrosylation
S685 Phosphorylation
T686 Phosphorylation
S688 Phosphorylation
T694 Phosphorylation

研究背景

機能:

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed by the liver and secreted in plasma.

サブユニット構造:

Monomer.

タンパク質ファミリー:

Belongs to the transferrin family.

研究領域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

参考文献

1). Withaferin A inhibits ferroptosis and protects against intracerebral hemorrhage. Neural Regeneration Research, 2023 (PubMed: 36453416) [IF=6.1]

Application: WB    Species: Mouse    Sample:

Figure 8 WFA induces HO-1 expression, resulting in ameliorated ferroptosis in hemin-induced SH-SY5Y neuroblast injury in vitro. (A) Representative western blot bands and (B) relative density of HO-1 in different groups. (C) Cell viability detection in different groups. (D) Typical BODIPY 581/591 C11 fluorescence images and (E) fluorescence density of BODIPY 581/591 C11 in different groups. Green indicates lipid ROS and blue indicates nuclei. (F) Representative western blot bands and (G–I) quantification of the relative density of 4-HNE, TF and FTH1 band densities in different groups. *P < 0.05, **P < 0.01 (one-way analysis of variance followed by Bonferroni correction). Data are expressed as the mean ± SEM (n = 3). 4-HNE: 4-Hydroxynonenal; FTH1: ferritin heavy chain 1; HO-1: heme oxygenase-1; TF: transferrin; WFA: withaferin A; ZnPP: zinc protoporphyrin, a specific HO-1 inhibitor.

Restrictive clause

 

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