FosB Antibody - #AF5010

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製品: FosB Antibody
カタログ: AF5010
タンパク質の説明: Rabbit polyclonal antibody to FosB
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Monkey
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
分子量: 35kDa; 36kD(Calculated).
ユニプロット: P53539
RRID: AB_2834932

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Monkey
予測:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
FosB Antibody detects endogenous levels of total FosB.
RRID:
AB_2834932
引用形式: Affinity Biosciences Cat# AF5010, RRID:AB_2834932.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Activator protein 1; AP 1; DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; fosB; FOSB_HUMAN; G0/G1 switch regulatory protein 3; G0S3; GOS 3; GOS B; GOS3; GOSB; MGC42291; Oncogene FOS B; Oncogene FOSB; Protein fos B; Protein fosB;

免疫原

免疫原:
Uniprot:

P53539(FOSB_HUMAN) >>Visit HPA database.

遺伝子(ID):
FOSB(2354)
タンパク質の説明:
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1.
タンパク質配列:
MFQAFPGDYDSGSRCSSSPSAESQYLSSVDSFGSPPTAAASQECAGLGEMPGSFVPTVTAITTSQDLQWLVQPTLISSMAQSQGQPLASQPPVVDPYDMPGTSYSTPGMSGYSSGGASGSGGPSTSGTTSGPGPARPARARPRRPREETLTPEEEEKRRVRRERNKLAAAKCRNRRRELTDRLQAETDQLEEEKAELESEIAELQKEKERLEFVLVAHKPGCKIPYEEGPGPGPLAEVRDLPGSAPAKEDGFSWLLPPPPPPPLPFQTSQDAPPNLTASLFTHSEVQVLGDPFPVVNPSYTSSFVLTCPEVSAFAGAQRTSGSDQPSDPLNSPSLLAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
89
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53539 基板として

Site PTM Type Enzyme
Y9 Phosphorylation
S11 Phosphorylation
S13 Phosphorylation
S27 Phosphorylation P68400 (CSNK2A1)
T151 Phosphorylation

研究背景

機能:

FosB interacts with Jun proteins enhancing their DNA binding activity.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Heterodimer.

タンパク質ファミリー:

Belongs to the bZIP family. Fos subfamily.

研究領域

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

参考文献

1). MAPK/AP-1 pathway regulates benzidine-induced cell proliferation through the control of cell cycle in human normal bladder epithelial cells. Oncology Letters, 2018 (PubMed: 30197677) [IF=2.9]

Application: WB    Species: human    Sample: SV‑HUC‑1

Figure 4. |Effects of benzidine on the expression and activation of mitogen‑activated protein kinases and activating protein‑1 monomers. (E) The Fos family, including p‑c‑Fos, FosB and Fra‑1, were all upregulated.

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