Slingshot homolog 1 Antibody - #DF13402
| 製品: | Slingshot homolog 1 Antibody |
| カタログ: | DF13402 |
| タンパク質の説明: | Rabbit polyclonal antibody to Slingshot homolog 1 |
| アプリケーション: | WB IHC |
| 反応性: | Human |
| 予測: | Bovine, Horse, Sheep, Dog |
| 分子量: | 115kDa; 116kD(Calculated). |
| ユニプロット: | Q8WYL5 |
| RRID: | AB_2846421 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13402, RRID:AB_2846421.
折りたたみ／展開
AW551225; Coro1c; coronin, actin binding protein 1C; Gm1394; Gm1395; hSSH-1L; mSSH 1L; OTTMUSP00000031415; Protein phosphatase Slingshot homolog 1; Slingshot 1; slingshot 1L; slingshot homolog 1; slingshot like 1; Slingshot1; SSH 1; SSH 1L; SSH-1L; SSH-like protein 1; Ssh1; SSH1_HUMAN;
免疫原
- Q8WYL5 SSH1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALVTLQRSPTPSAASSSASNSELEAGSEEDRKLNLSLSESFFMVKGAALFLQQGSSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESAWADRVRYMVVVYSSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKELKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQTDSSLQQPVDDPAGPGDFLPETPDGTPESQLPFLDDAAQPGLGPPLPCCFRRLSDPLLPSPEDETGSLVHLEDPEREALLEEAAPPAEVHRPARQPQQGSGLCEKDVKKKLEFGSPKGRSGSLLQVEETEREEGLGAGRWGQLPTQLDQNLLNSENLNNNSKRSCPNGMEDDAIFGILNKVKPSYKSCADCMYPTASGAPEASRERCEDPNAPAICTQPAFLPHITSSPVAHLASRSRVPEKPASGPTEPPPFLPPAGSRRADTSGPGAGAALEPPASLLEPSRETPKVLPKSLLLKNSHCDKNPPSTEVVIKEESSPKKDMKPAKDLRLLFSNESEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSVPADPAPPSRDGPASRLEASIPEESQDPAALHELGPLVMPSQAGSDEKSEAAPASLEGGSLKSPPPFFYRLDHTSSFSKDFLKTICYTPTSSSMSSNLTRSSSSDSIHSVRGKPGLVKQRTQEIETRLRLAGLTVSSPLKRSHSLAKLGSLTFSTEDLSSEADPSTVADSQDTTLSESSFLHEPQGTPRDPAATSKPSGKPAPENLKSPSWMSKS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8WYL5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T5 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| T403 | Phosphorylation | Uniprot | |
| Y407 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| T430 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| Y443 | Phosphorylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| T462 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| T526 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| T590 | Phosphorylation | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| Y646 | Phosphorylation | Uniprot | |
| K758 | Methylation | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| T826 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S834 | Phosphorylation | Uniprot | |
| S854 | Phosphorylation | Uniprot | |
| S889 | Phosphorylation | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| S897 | Phosphorylation | Uniprot | |
| T908 | Phosphorylation | Uniprot | |
| S910 | Phosphorylation | Uniprot | |
| T922 | Phosphorylation | Uniprot | |
| T933 | Phosphorylation | Uniprot | |
| S935 | Phosphorylation | Uniprot | |
| S936 | Phosphorylation | Uniprot | |
| S937 | Phosphorylation | Q9BZL6 (PRKD2) , Q15139 (PRKD1) , Q13557 (CAMK2D) | Uniprot |
| S938 | Phosphorylation | Uniprot | |
| S940 | Phosphorylation | Uniprot | |
| S943 | Phosphorylation | Uniprot | |
| K952 | Acetylation | Uniprot | |
| S970 | Phosphorylation | Uniprot | |
| S971 | Phosphorylation | Uniprot | |
| S978 | Phosphorylation | Q13557 (CAMK2D) , Q15139 (PRKD1) , Q9BZL6 (PRKD2) | Uniprot |
| T1021 | Phosphorylation | Uniprot | |
| K1041 | Methylation | Uniprot | |
| S1042 | Phosphorylation | Uniprot |
研究背景
Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.
Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium. Cleavage furrow. Midbody.
Note: Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
Interacts with actin and this stimulates phosphatase activity. Also interacts with LIMK1 and with the 14-3-3 proteins YWHAB, YWHAG, YWHAQ, and YWHAZ. Interaction with 14-3-3 proteins inhibits phosphatase activity and also blocks recruitment to lamellipodia and stimulation by actin.
Belongs to the protein-tyrosine phosphatase family.
研究領域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
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